Cloning and Molecular Phylogenetic Analysis of p10 Gene from Bombyx mori Nucleopolyhedrovirus

According to the sequence of p10 gene of Bombyx mori nuclear polyhedrosis virus(BmNPV) isolate T3,eight p10 genes of BmNPV collected from different areas were cloned and aligned respectively with relevant sequences in GenBankTM database.The open reading frame(ORF) of all p10 genes from the 8 strains of BmNPVs include 213 nucleotides,encoding a putative protein of 70 amino acids.Multiple alignment of the P10 proteins indicated that the similarities between the 8 strains are 95%-100%.The p10 genes in all of the 8 strains of BmNPVs also lacked the C-terminal structure domain when compared with AcMNPV p10 gene and BmNPV p10 gene.Therefore,the p10 genes cloned only contains two conserved domains,namely the N-terminal coiled-coil domain,and a proline-rich domain.