Effects of different treatments on yeast cell membrane permeability

In this project, the effect of ultrasounding treatment with three sorts of organic solvents on yeast cell membrane permeability was studied, by measuring the concentration of nucleic acid, protein and fructose-1,6-diphosphate (FDP) before and after the treatment. The result showed that by increasing the effective time, the concentration of nucleic acid , protein and FDP in the broth increased gradually after ultrasound treatment with power of 600, 500 and 400 W, the most notable effect being achieved by 600 W ultrasounding. After treatment with those ultrasounds, the livability of cells decreased. That was to say, the number of disintegrated cells increased. With the extension of effective time, livability decreased most quickly when applying 600 W, then 500 W and 400 W. Among organic solvents, toluol and chloroform affected exudation from the cell very much, but damage the cell severely. Aether has little influence on the cell livability.