Sequence Analysis of gE Antigen Epitope Genes of Pseudorabies Virus

A pair of primers were designed and synthesized. Using the whole genomes of strain PVR MinA and PRV YueA as templates,specific products were obtained as expected by PCR amplification and cloned into pUCm T vector.Recombinants were confirmed by colony PCR and restriction enzyme digestion.The inserts were sequenced,and the results revealed that each of both inserts composed of 558 neucleotides,coding for 186 amino acids.Comparison of the target genes showed 96 9% nuleotide sequence homology and 92.5% amino acid sequence homology between strain PRV YueA and PRV MinA,which is relatively low.