Studies on the Condition of Phytase Produced by Penicillium and Its Purification

HE Ping 1,FU Xue lin 2,ZHAN Fu jian 1,ZHAO Ya hua 1,GAO Xiang yang 1,XU Shao jie 1

doi:10.7671/j.issn.1001-411X.2002.02.013

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Journal of South China Agricultural University > 2002 > 23(2): 44-46. > DOI: 10.7671/j.issn.1001-411X.2002.02.013

HE Ping 1,FU Xue lin 2,ZHAN Fu jian 1,ZHAO Ya hua 1,GAO Xiang yang 1,XU Shao jie 1. Studies on the Condition of Phytase Produced by Penicillium and Its Purification[J]. Journal of South China Agricultural University, 2002, 23(2): 44-46. DOI: 10.7671/j.issn.1001-411X.2002.02.013

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Studies on the Condition of Phytase Produced by Penicillium and Its Purification

HE Ping 1,FU Xue lin 2,ZHAN Fu jian 1,ZHAO Ya hua 1,GAO Xiang yang 1,XU Shao jie 1

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Revised Date: September 17, 2001

Abstract

Abstract

When the original pH value of the penicillium's culture fluid was 5.5,the phytase was produced much .While the pH of culture fluid didn't decrease, the growth of penicillium could be inhibited. Calcium phytate could promote the penicillium to increase phytase activities.Two isoenzymes of phytase from penicillium were accepted by ammonium sulfate precipitation,sephadex G-100 gel filtration chromatography. The purification factors of phytase reached 3.3 folds with a specific activity of 71 3 U/mg.
- penicillium,
- phytate,
- phytase