Improved Method of PEG Embedding Section Being Used in Investigating Microtubules in Plant Embryo Sac

Improved PEG embedded section method was described. The method made it easy to operate and the results were obviously clearer. Ovaries of rice were conducted by fixing, dehydrating, embedding by PEG in small centrifuge tube, cutting section by razor, vacuum gluing section, labeling by fluorescence antibody, and then microtubules in embryo sac were observed and photographed by confocal laser scanning microscopy. The former gluing section methods (such as poly lysine gluing section method) blocked severely the conjuncting between microtubules and antibodies, vacuum gluing section method being founded by us resolved the problem, the new gluing section method was the main reason leading to improvement of results. One most obvious feature of vacuum gluing section method was without sticker. The method resolved all problems caused by sticker, and the method was suitable for the situation that needed gluing section but sticker blocked the later conducting,and the method was possible to be widely used in other researches.