Sequence Analysis of cDNA of the Gene Coding for VP5 and Noncoding Regions of Infectious Bursal Disease Virus

A cDNA fragment coding for VP5 and noncoding regions of three strains of IBDV were amplified by reverse transcription polymerase chain reaction (RT PCR) from the genomic dsRNA of CJ801, LX, HK46 respectively. The amplified cDNAs of three strains were cloned into the plasmid pBssk to generate recombinant plasmids, and to be used for sequencing. This experiment is the report about the sequence of VP5 and noncoding regions of CJ801 and LX. The VP5 of most strains consist of 145 AA and has only one hydrophilic region, whereas the VP5 of vvIBDV and variant strains consist of 149 AA and has two hydrophilic regions, which may contribute to virulence variation.