Transcriptome analysis of Drosophila S2 cells after infection of Listeria monocytogenes, L. grayi or L. welshimeri

DENG Jianhao; LIU Li; SHU Chan; WU Kun; WU Kunzhong; YAO Binghua; LI Kang; TIAN Ling; HUANG Zhijun

doi:10.7671/j.issn.1001-411X.202312026

Journal of South China Agricultural University > 2025 > 46(1): 12-24. > DOI: 10.7671/j.issn.1001-411X.202312026

DENG Jianhao, LIU Li, SHU Chan, et al. Transcriptome analysis of Drosophila S2 cells after infection of Listeria monocytogenes, L. grayi or L. welshimeri[J]. Journal of South China Agricultural University, 2025, 46(1): 12-24. DOI: 10.7671/j.issn.1001-411X.202312026

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Transcriptome analysis of Drosophila S2 cells after infection of Listeria monocytogenes, L. grayi or L. welshimeri

1.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China
2.
Guangmeiyuan R & D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China

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Received Date: December 19, 2023
Available Online: December 16, 2024
Published Date: September 25, 2024

Graphical Abstract

Abstract

Abstract

Objective
To compare the gene expression patterns in S2 cells after infection of Listeria monocytogenes, L. grayi, and L. welshimeri using Drosophila as a host, and provide a research basis for exploring the innate immune defense mechanisms of the host and the differential pathogenic mechanisms of different Listeria strains.
Method
After infection of L. monocytogenes, L. grayi or L. welshimeri for 3 h, the mRNA expression profiles in Drosophila S2 cells were detected using transcriptome sequencing technology. Analysis of differentially expressed genes (DEGs) was performed using bioinformatics tools of GO annotation and KEGG analysis. Finally, the mRNA levels of five antimicrobial peptide genes associated with the Toll and Imd signaling pathways were validated using qRT-PCR technology.
Result
After infection of L. monocytogenes, L. gray or L. welshimeri, there were 18 DEGs in common, as well as 104, 28, and 33 unique DEGs in the Drosophila S2 cells respectively. The DEGs commonly existed in three Listeria infected groups were annotated in GO terms including metabolic process, cellular process, organelle, response to stimulus, immune system process and etc. The unique DEGs existed in L. monocytogenes infected group were annotated in GO terms such as biological adhesion, presynaptic process involved in chemical synaptic transmission, negative regulation of biological process, nucleic acid binding transcription factor activity, signal transducer activity, and etc. The unique DEGs in L. grayi infected group were annotated in GO terms such as multi-organism process and extracellular regions, while the unique DEGs in L. welshimeri infected group were annotated in GO terms such as developmental processes and membrane-enclosed lumen. The KEGG analysis results showed that the DEGs of three Listeria infected groups were enriched in the Toll/Imd signaling pathways. The quantitative results of qRT-PCR validation of five antimicrobial peptide genes related to Toll and Imd signaling pathways were consistent with the transcriptomic results.
Conclusion
The expression patterns in S2 cells affected by the infection of these three Listeria species exhibited some similarities but also differences. The variation of the signaling pathways may be related to the virulence of these Listeria species in S2 cells.
- Listeria monocytogenes,
- Listeria gray,
- Listeria welshimeri,
- Drosophila S2 cell,
- Transcriptome sequencing,
- Antimicrobial peptide,
- Signaling pathway

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References (36)

References

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Transcriptome analysis of Drosophila S2 cells after infection of Listeria monocytogenes, L. grayi or L. welshimeri

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