Intervention of mannan oligosaccharide on fatty liver syndrome  in Roman laying hens fed high-fat diet

ZHAO Weijie; FENG Xiaohua; LIANG Jingwen; MO Yingfen; ZHU Canjun; SHU Gang; GAO Ping; WANG Songbo; WANG Lina; JIANG Qingyan

doi:10.7671/j.issn.1001-411X.202209038

Journal of South China Agricultural University > 2024 > 45(1): 15-22. > DOI: 10.7671/j.issn.1001-411X.202209038

ZHAO Weijie, FENG Xiaohua, LIANG Jingwen, et al. Intervention of mannan oligosaccharide on fatty liver syndrome in Roman laying hens fed high-fat diet[J]. Journal of South China Agricultural University, 2024, 45(1): 15-22. DOI: 10.7671/j.issn.1001-411X.202209038

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Intervention of mannan oligosaccharide on fatty liver syndrome in Roman laying hens fed high-fat diet

College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Nutrition Control, Guangzhou 510642, China

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Received Date: September 25, 2022
Available Online: November 21, 2023
Published Date: August 28, 2023

Graphical Abstract

Abstract

Abstract

Objective
The purpose of this study was to investigate the effect of dietary supplementation of mannose oligosaccharide (MOS) on fatty liver syndrome and intestinal flora of Roman laying hens.
Method
Eighty 230-day-old Roman laying hens were divided into four groups with 20 hens in each group, including the normal diet group (CK group), high-fat diet group (HFD group), HFD+0.1% MOS group (0.1% MOS group), and HFD+0.5% MOS group (0.5% MOS group). Samples of serum, liver, abdominal fat and intestinal contents were taken at the 9th week of the experiment. The regulatory effect of MOS on fatty liver in Roman hens was investigated by analyzing the serum indicators and gene expression in liver and gut microbiota sequencing.
Result
High-fat diet significantly increased body weight, liver weight, liver index, abdominal fat weight and abdominal fat index of Roman laying hens, and induced fatty liver in laying hens. MOS significantly reduced the increase of body weight, liver weight and abdominal fat weight of laying hens caused by HFD. MOS significantly inhibited the increase of fat deposition in the serum and liver of laying hens caused by HFD. MOS inhibited mRNA expression levels of fat synthesis genes ACC, FAS and SCREBP-1c in liver, and alleviated fatty liver of laying hens. MOS significantly increased the relative abundance of Bacteroidetes and decreased the relative abundance of Firmicutes in cecum of laying hens.
Conclusion
Dietary supplementation of MOS in HFD can improve the gut microbiome of laying hens, and inhibit mRNA expression levels of genes related to fat synthesis in liver, thereby alleviating the fatty liver of laying hens caused by HFD. Addition of 0.5% MOS in diet has a better effect for reducing the body weight, liver weight and abdominal fat weight of laying hens.
- Mannose oligosaccharide,
- High-fat diet,
- Roman laying hen,
- Fatty liver syndrome,
- Intestinal flora

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References (29)

References

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Intervention of mannan oligosaccharide on fatty liver syndrome in Roman laying hens fed high-fat diet

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