| Citation: |
GUO Tao, SHEN Renjia, WANG Jiafeng. Research progress on genetic transformation methods of rice[J]. Journal of South China Agricultural University, 2023, 44(6): 843-853. DOI: 10.7671/j.issn.1001-411X.202307001
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GUO Tao: 郭 涛，研究员，博士生导师。现任国家植物航天育种工程技术研究中心主任，航天育种产业创新联盟副秘书长，国家植物航天育种工程技术研究中心河源创新研究院院长。基于新一代航天工程，开展水稻空间生物学研究，解析空间环境与遗传进化的分子关联，并开发水稻定向诱变育种新技术；结合分子标记和复合杂交，创制多基因聚合优异材料。获授权水稻生物育种关键技术专利12件；第一完成人培育水稻新品种6个；发表各类论文62篇，2篇论文入选中国精品科技期刊顶尖学术论文；现为国家重点研发计划课题负责人和国际原子能机构CRP项目首席科学家，并参与省部级科研项目26项。先后获广东省科技进步奖、教育部优秀成果奖、广东省专利奖、广东省农业技术推广奖等省部级科研奖励6项
To introduce the development process and achievements of rice genetic transformation methods, and provide references for the research and application of rice genetic transformation system. Based on the two methods of bio-mediated transformation and abiotic-mediated transformation, the first report and important progress of various transformation methods were introduced, with the prospect at last. Among the bio-mediated transformation methods, Agrobacterium-mediated transformation method is applied by infecting rice embryo, panicle, callus tissue and stem tip. The transformation system of seed embryo and its induced callus as materials is mature, and the transformation method using panicle and stem tip is simple and convenient, with short transformation and regeneration cycle. In addition, preliminary studies have tried to transform rice with Sinorhizobium, Rhizobium and Ensifer adhaerens. Among the abiotic-mediated transformation methods, physical method transformation methods (particle bombardment, electroporation, pollen-tube pathway and microinjection) are more traditional transformation methods. The particle bombardment method is relatively mature, and the pollen-tube pathway method has made great progress in breeding. Among the medium-mediated transformation methods, the application of nanomaterials is gradually becoming a research hotspot. The development of rice genetic transformation can be initiated by selecting transformation materials and optimizing transformation vectors. Concurrently, integrating the transformation system with techniques like DNA-free and haploid induction can enhance both transformation efficiency and safety, while also reducing the duration of the transformation and regeneration process.
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