Abstract: The culture of the nodal segments from juyetnile and mature shoots of Clausena lanstum (Lour) skeels grown in field was successfully established in vitro. The suitable medium was WPM + BA 1.0+KT 0.5+NAA 0.5 + GA3 0.25+ 4-5% sucrose. Surfaces of plant parts carry a wide range of microbial contamints, which were the main sourse of infection to plant in vitro.