Abstract: In order to determinate combining ability of three-line hybrid sows, we compared growth performance, carcass performance and body conformation of four kinds of hybrid sows, including W211, W212, W351 and W352 which were produced by W51, W52, W61 and W62 lines, and four kinds of hybrid sows were mated with the S122 boar to produce commercial pigs in Zhagang pig farm of Huaiji branch of Guiyue company. The reproductive performance of different hybrid sows were compared. The results showed that there were some differences among them in terms of body conformation. The body length of W211 pig was short, teat number was less, back fat was thin and growth rate was medium. The effective teat number of W212 pig was more, body length was medium, back fat was thicker and growth rate was faster. The effective teat number of W351 pig was less, body length was longer, back fat was medium and growth rate was slower. The body length of W352 pig was the longest, effective teat number was more, back fat was medium and growth rate was faster. W352 sows had obvious advantages in terms of total litter size, number born alive, number born robust, and litter weight at the age of 21 days, and the heterosis rate of its hybrid combination was higher. Among four kinds of hybrid sows, W352 pig had a longer body length with obvious advantages of reproductive performance.