摘要:
【目的】从目前已知的参与拟南芥 Arabidopsis thaliana 次生壁加厚生长的转录因子着手，分析这些次生壁相关的转录因子是否能够调控木糖合成关键酶基因 FRA8、IRX9、IRX10、IRX14、F8H、IRX9-L、IRX10-L 和 IRX14-L 的表达,并且观察 KNAT7 基因显性抑制植株的表型.【方法】通过 Gateway 技术构建效应器和报告器，进行瞬时转录激活试验,同样构建 pCAMBIA1304-p35S∷ KNAT7-SRDX 重组质粒，用农杆菌Agrobacterium tumefaciens花序浸染法将此质粒转化到野生型拟南芥植株中.【结果和结论】瞬时转录激活试验表明,转录因子 KNAT7、MYB46、ERF72、SND1、NST2 能够激活多个拟南芥木聚糖合成关键酶基因的表达，其中KNAT7能促进基因 FRA8、IRX9 和 IRX14-L 的表达. KNAT7 基因显性抑制能显著影响拟南芥的生长.试验结果表明 KNAT7 基因可能在木聚糖的合成中起着重要的调控作用.
Abstract:
【Objective】 To analyze whether some transcription factors in Arabidopsis thaliana, known for the secondary cell wall thicken, could regulate the expression of the key genes of xylosyltransferase, such as FRA8, IRX9, IRX10, IRX14, F8H, IRX9-L, IRX10-L and IRX14-L, and observe the phenotype of KNAT7 dominant repression plant.【Method】 Effectors and reporters were constructed by Gateway Technology and the transient transcriptional activation assay was conducted. Construct pCAMBIA1304-p35S∷KNAT7-SRDX recombinant plasmid by Gateway Technology and transform this plasmid into wild A. thaliana via Agrobacterium tumefaciens-floral dip method．【Result and conclusion】 The transient transcriptional activation assay revealed that transcription factors KNAT7, MYB46, ERF72, SND1, NST2 could activate the expression of a number of the key genes of xylosyltransferase. KNAT7 could activate the expression of FRA8, IRX9 and IRX14-L. Furthermore, dominant repression of KNAT7 significantly affected the growth of Arabidopsis thaliana. These results indicate that KNAT7 probably plays an important role in the regulation of xylan biosynthesis.
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