甲硝唑半抗原及全抗原合成与鉴定

基金项目: 国家自然科学基金 , 广东省自然科学基金 , 粤港关键领域重点突破项目

Synthesis and Identification of the Hapten and Complete Antigen of Metronidazole

1 .The Higher Education Key Lab of Guangdong Province - Food Quality and Safety Laboratory; Institute of Food Quality and Safety, South China Agric. Univ. , Guangzhou 510642, China; 2 .College of Veterinary Medicine, South China Agric. Univ. , Guangzhou 510642, China

摘要: 在微波加热条件下,将甲硝唑与琥珀酸酐反应,合成半抗原甲硝唑半琥珀酸酯,氢核磁共振、电喷雾质谱、红外光谱方法的鉴定结果表明,目标半抗原合成成功;通过活泼酯法将半抗原与载体蛋白偶联制备甲硝唑全抗原,紫外光谱法鉴定结果表明,全抗原合成成功.
- 甲硝唑 /
- 半抗原 /
- 全抗原 /
- 鉴定 /
- 甲硝唑 /
- 半抗原合成 /
- Metronidazole /
- Antigen /
- Complete /
- Hapten /
- Identification /
- 紫外光谱法 /
- 全抗原 /
- 蛋白偶联 /
- 载体 /
- 活泼酯 /
- 目标 /
- 鉴定结果 /
- 红外光谱方法 /
- 喷雾质谱 /
- 氢核磁共振 /
- 琥珀酸酯 /
- 酸酐反应 /
- 加热条件
Abstract: The hapten metronidazole monosuccinate was synthesized by reacting metronidazole and succinic anhydride under microwave heating. Identification using 1^H nuclear magnetic resonance, electrospray ionization mass and infrared spectrum showed the hapten was successfully synthesized. Then active ester method was used to produce complete antigen of metronidazole, which was confirmed by analysis using ultraviolet spectrum.
- metronidazole /
- hapten /
- complete antigen /
- identification