张守全,王翀,杨关福,戴文滔. 巢式RT-PCR检测昆明小鼠早期胚胎中G6PD基因表达[J]. 华南农业大学学报, 1999, (1): 21-25.
    引用本文: 张守全,王翀,杨关福,戴文滔. 巢式RT-PCR检测昆明小鼠早期胚胎中G6PD基因表达[J]. 华南农业大学学报, 1999, (1): 21-25.
    Zhang Shouquan,Wang Chong,Yang Guanfu,Dai Wentao. Expression of G6PD Gene by Nested RT-PCR inKunming Mouse Early Embryos[J]. Journal of South China Agricultural University, 1999, (1): 21-25.
    Citation: Zhang Shouquan,Wang Chong,Yang Guanfu,Dai Wentao. Expression of G6PD Gene by Nested RT-PCR inKunming Mouse Early Embryos[J]. Journal of South China Agricultural University, 1999, (1): 21-25.

    巢式RT-PCR检测昆明小鼠早期胚胎中G6PD基因表达

    Expression of G6PD Gene by Nested RT-PCR inKunming Mouse Early Embryos

    • 摘要: 根据小鼠肌肉的6-磷酸葡萄糖脱氢酶(G6PD)的cDNA序列设计并合成内外2对引物,以巢式RT-PCR方法检测昆明小鼠1-,2-,4-,8-细胞期早期胚盈是否转录出G6PD的mRNA,结果发现1 ̄8-细胞期早期胚胎的mRNA均能以外引物扩增产物为模板通过内引物扩增出一条378bp特异性条带。表明昆明小鼠1 ̄8-细胞期早期胚胎的G6PD基因已表达,磷酸戊糖途径已是其代谢葡萄糖的主要方式之一。

       

    /

    返回文章
    返回