Abstract: Successful veterinary application of 3%hydrogen peroxide solution instead of con-ventional co ntrast agents for echocardiographic acoustic contrast is reported in this paper for the first time.Comparative tests using different kinds of contrast agents con-firmed that the 3 % H2O2 solution used for echocardiographic acoustic contrast in bovines was safe and reliable,simple and easy,with good reproducibility.The sonographic presentation of 3% H2O2 was markedly superior to that of sodium chlo-ride injection and 5 % glucose solution.