Abstract: Cordyceps brasiliensis was collected in Guandong Province and inoculated into pupae ofBombyx mori.,and artificial culture of Cordyceps brasiliensis was successfully achieved.Themethod of artificial culture using the pupa of Bombyx mori.is described in this paper.Theergosterol,mannitol,adenosine,amino and microelement content of the artificially cul-tured Cordyceps brasiliensis was similar to that of Cordyceps sinensis.It had thepharmacologic effect of enhancing immunity and decreasing serum cholesterol in experimentalanimals.