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    李德望 唐伟文. 柑桔黄龙病的血清学检测与诊断方法的初步研究[J]. 华南农业大学学报, 1992, (2): 16-22.
    引用本文: 李德望 唐伟文. 柑桔黄龙病的血清学检测与诊断方法的初步研究[J]. 华南农业大学学报, 1992, (2): 16-22.
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    Li Dewang Tang Weiwen Faan Hweichung. PRELIMARY STUDIES ON THE METHODS OF RAPID SEROLOGICAL DETECTION AND DIAGNOSIS OF THE BLO ASSOCIATED WITH CITRUS SHOOT-YELLOWING[J]. Journal of South China Agricultural University, 1992, (2): 16-22.
    Citation: Li Dewang Tang Weiwen Faan Hweichung. PRELIMARY STUDIES ON THE METHODS OF RAPID SEROLOGICAL DETECTION AND DIAGNOSIS OF THE BLO ASSOCIATED WITH CITRUS SHOOT-YELLOWING[J]. Journal of South China Agricultural University, 1992, (2): 16-22.
    shu

    柑桔黄龙病的血清学检测与诊断方法的初步研究

    PRELIMARY STUDIES ON THE METHODS OF RAPID SEROLOGICAL DETECTION AND DIAGNOSIS OF THE BLO ASSOCIATED WITH CITRUS SHOOT-YELLOWING

      摘要
    • 摘要: 用提纯的柑桔黄龙病病原(CYSO)免疫小鼠制备出抗体IgG,其浓度与2.7mg/ml,并证明它对CYSO具有特异性,效价测定结果:ELISA达1∶640,间接免疫荧光(IIF)达1∶1 000;斑点(?)联免疫技术(Dot-ELISA)达1∶1280;斑点免疫荧光技术(Dot-IF)达1∶1 280,检测纯化抗原(?)度,ELISA达1∶256;IIF达1∶4 096,Dot-ILISA达1∶8 192,Dot-IF达1∶32 768。ELISA只能用于检测典型的蕉柑和椪柑病材料,面IIF,dot-ILISA和Dot-IF还可用于甜橙的检测,用4种方法均不能检测出未显症材料的CYSO。

       

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