Abstract: In order to overcome the difficulties in radiography of the bovine lumbar vertebrae, studies were carried out using artificial pneumoperitoneum in combination with an intrarectal cassette. The major results obtained were as follows:The main structure of L1-L5 could be visualized clearly in the right lateral radiographs of the lumbar vertebrae after creating artificial pneumoperitontum.Grades of well defined(1)and evident (2) were given to 92%(23/25) of the radiographs.No grade (1)or (2) was present in the control radiographs taken before artificial pneumoperitoneum, and grades of poor (3) and not visualized (4) were given to 100%(25/25)of them.The main structure of the lumbar vertebrae could not be visualized clearly even not at all in the control radiographs. The main, structure of 15-S1 was visualized clearly in the dorsovential radiographs of the lumbosacral region made by means of intrarectal cassette.Grades of well defined(1)and evid-ent(2) were given to 89.24%(15/17)of them.The results showed that the inherent difficulty of bovine lumbar vertebrae radiography could be overcome by means of using artificial pneumoperitoteum, which makes it possible to do a diagnostic radiography of the bovine lumbar vertebrae with the use of ordinary X-ray apparatus.The safety of the pneumoperitotieum was also studied and discussed.