麦桂婉, 肖文超, 李云锋, 等. 不同类型香蕉的细菌性鞘腐病病原分子鉴定及致病性分析[J]. 华南农业大学学报, 2024, 45(4): 535-541. doi: 10.7671/j.issn.1001-411X.202312018
    引用本文: 麦桂婉, 肖文超, 李云锋, 等. 不同类型香蕉的细菌性鞘腐病病原分子鉴定及致病性分析[J]. 华南农业大学学报, 2024, 45(4): 535-541. doi: 10.7671/j.issn.1001-411X.202312018
    MAI Guiwan, XIAO Wenchao, LI Yunfeng, et al. Molecular identification and pathogenicity analysis of bacterial sheath rot pathogen in different types of banana[J]. Journal of South China Agricultural University, 2024, 45(4): 535-541. doi: 10.7671/j.issn.1001-411X.202312018
    Citation: MAI Guiwan, XIAO Wenchao, LI Yunfeng, et al. Molecular identification and pathogenicity analysis of bacterial sheath rot pathogen in different types of banana[J]. Journal of South China Agricultural University, 2024, 45(4): 535-541. doi: 10.7671/j.issn.1001-411X.202312018

    不同类型香蕉的细菌性鞘腐病病原分子鉴定及致病性分析

    Molecular identification and pathogenicity analysis of bacterial sheath rot pathogen in different types of banana

    • 摘要:
      目的 香蕉Musa spp.是我国南方重要的经济作物,香蕉细菌性鞘腐病是近年来发现的一种细菌性病害,给香蕉生产带来严重影响。本研究旨在明确侵染不同类型香蕉的病原种类和致病性分化。
      方法 对多种不同类型香蕉的细菌性鞘腐病病株进行分离,并利用Dickeya属特异引物对这些病原进行PCR鉴定;通过分析16S rDNA核苷酸序列相似性、构建6个管家基因(fusAgyrArecAgyrBdnaJdnaX)的系统进化树和分析共线性关系进一步确定病原。采用离体和活体接种香蕉假茎及幼苗对所分离病原进行致病性分析。
      结果 从不同类型香蕉病株中共分离获得15株菌株,PCR鉴定表明这些病原属于Dickeya spp.。16S rDNA核苷酸序列分析、基于6个管家基因的系统进化分析以及共线性分析的结果表明,所分离的菌株属于D. dadantii。致病性测定结果表明,这15株菌株致病性分为3类:XJ1、XJ8、XJ12和对照XJ5-1属于强致病力菌株,XJ2、XJ3、XJ4、XJ7和XJ11是较强致病力菌株,XJ5、XJ6、XJ9、XJ10和XJ13为中等致病力菌株。
      结论 来源于不同类型香蕉的细菌性鞘腐病病原均为D. dadantii,但是不同菌株间存在明显的致病性分化,为进一步研究D. dadantii的致病机理以及香蕉细菌性鞘腐病的防控奠定了理论基础。

       

      Abstract:
      Objective Banana (Musa spp.) is an important ecomomic crop in South China, banana bacterial sheath rot disease has been found in recent years, which severely affects banana production. This study aimed to clarify the pathogens from different types of bananas and their pathogenicity differentiation.
      Method The pathogens of bacterial sheath rot were isolated from different types of bananas, and were identified by PCR using Dickeya genus-specific primers. The pathogens were further confirmed by analyzing the nucleotide sequence similarity of 16S rDNA, constructing the phylogenetic analysis based on six housekeeping genes (fusA, gyrA, recA, gyrB, dnaJ, and dnaX), and analyzing the collinear relationship. The pathogenicity of isolated pathogens were analyzed by in vitro and in vivo inoculating pseudostems and seedlings of banana.
      Result Fifteen strains were isolated from different types of bananas and identified as Dickeya spp. by PCR. All fifteen strains were confirmed as D. dadantii according to the results of 16S rDNA nucleotide sequences analysis, the phylogenetic analysis based on the six housekeeping genes and the collinearity analysis. The pathogenicity analysis showed that the pathogenicity of fifteen strains could be classified into three types, the strong pathogenic strains included XJ1, XJ8, XJ12 and control XJ5-1, the relatively strong pathogenic strains included XJ2, XJ3, XJ4, XJ7 and XJ11, and the moderate pathogenic strains included XJ5, XJ6, XJ9, XJ10 and XJ13.
      Conclusion All the pathogens causing bacterial sheath rot from different types of bananas belong to D. dadantii, yet there is obvious pathogenic differentiation among them, which provides a basis for future research on the pathogenic mechanisms of D. dadantii and the prevention and control of banana bacterial sheath rot.

       

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