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    李锦, 谭德东, 邱济平, 等. 香蕉抗枯萎病株系内生菌的分离鉴定及抑菌促生效果[J]. 华南农业大学学报, 2024, 45(2): 256-265. doi: 10.7671/j.issn.1001-411X.202308003
    引用本文: 李锦, 谭德东, 邱济平, 等. 香蕉抗枯萎病株系内生菌的分离鉴定及抑菌促生效果[J]. 华南农业大学学报, 2024, 45(2): 256-265. doi: 10.7671/j.issn.1001-411X.202308003
    shu
    LI Jin, TAN Dedong, QIU Jiping, et al. Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects[J]. Journal of South China Agricultural University, 2024, 45(2): 256-265. doi: 10.7671/j.issn.1001-411X.202308003
    Citation: LI Jin, TAN Dedong, QIU Jiping, et al. Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects[J]. Journal of South China Agricultural University, 2024, 45(2): 256-265. doi: 10.7671/j.issn.1001-411X.202308003
    shu

    香蕉抗枯萎病株系内生菌的分离鉴定及抑菌促生效果

    Isolation and identification of endophytic bacteria from banana fusarium wilt resistant strains and their inhibitory and growth-promoting effects

      摘要
    • 摘要:
      目的 筛选和鉴定从威廉斯香蕉抗枯萎病株系根、球茎及假茎分离得到的可抑制香蕉枯萎病菌(Fusarium oxysporum f. sp. cubense，Foc)内生菌，研究其抑菌促生效果。
      方法 通过16S rRNA基因序列测定和生理生化试验，对香蕉抗枯萎病株系内生菌进行种类鉴定和系统发育分析；通过平板对峙试验检测各菌株的抑菌能力，探究拮抗菌分泌抑菌相关活性物质的种类；采用盆栽试验对香蕉抗枯萎病株系内生菌进行生防功能和促生功能的分析，筛选出生防性能优良且兼具促生效果的内生菌，并在大田试验中验证。
      结果 分离获得13株代表菌株，其中8株对枯萎病1号生理小种(Foc 1)具有抑菌活性，7株对枯萎病4号生理小种(Foc 4)具有抑菌活性；综合盆栽试验和大田试验中代表菌株的防治效果及促生能力，筛选出贝莱斯芽孢杆菌XG1028、变栖克雷伯氏菌XQJ0301和暹罗芽孢杆菌XG0103共3株具备高抑菌活性以及促生能力的生防菌株。
      结论 本研究筛选出的3株菌株丰富了抗香蕉枯萎病微生物种质资源，为防治香蕉枯萎病寻找安全、环保的新型生物防治剂提供参考。

       

      Abstract:
      Objective To screen and identify endophytic bacteria that can inhibit Fusarium oxysporum f. sp. cubense (Foc) isolated from roots, bulbs and pseudostems of wilt-resistant strains of Williams banana, and study their inhibitory and growth-promoting effects.
      Method Through 16S rRNA gene sequence and physiological and biochemical tests, species identification and phylogenetic analysis of endophytic bacteria in banana fusarium wilt resistant strains were carried out. The plate confrontation test was carried out to detect the antibacterial ability of strain and explore the types of antibacterial related active substances secreted by antagonist. The biocontrol and growth-promoting functions of endophytic bacteria in banana resistant strains to wilt disease were analyzed by pot experiment. The endophytes with good biocontrol and growth promoting effects were selected and verified in field.
      Result Thirteen representative strains were isolated, among which, there were 8 strains with antibacterial activity against Foc 1 and 7 strains with antibacterial activity against Foc 4. Three biocontrol strains with high inhibitory activity and growth promotion ability were selected by the comprehensive results of biocontrol and growth promoting effects in pot and field experiments, including Bacillus velezensis XG1028, Klebsiella mutans XQJ0301 and Bacillus siamensis XG0103.
      Conclusion Three screened strains can enrich the germplasm resources of microorganisms resistant to banana wilt disease, and provide a reference for finding the new safe and environmentally friendly biological control agents controlling banana wilt disease.

       

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