王琦, 杜庆鑫, 孟益德, 等. 不同良种杜仲叶主要营养成分的动态变化与综合评价[J]. 华南农业大学学报, 2024, 45(4): 1-11. doi: 10.7671/j.issn.1001-411X.202306023
    引用本文: 王琦, 杜庆鑫, 孟益德, 等. 不同良种杜仲叶主要营养成分的动态变化与综合评价[J]. 华南农业大学学报, 2024, 45(4): 1-11. doi: 10.7671/j.issn.1001-411X.202306023
    WANG Qi, DU Qingxin, MENG Yide, et al. Dynamic change and comprehensive evaluation of main nutrients in leaves of different Eucommia ulmoides improved varieties[J]. Journal of South China Agricultural University, 2024, 45(4): 1-11. doi: 10.7671/j.issn.1001-411X.202306023
    Citation: WANG Qi, DU Qingxin, MENG Yide, et al. Dynamic change and comprehensive evaluation of main nutrients in leaves of different Eucommia ulmoides improved varieties[J]. Journal of South China Agricultural University, 2024, 45(4): 1-11. doi: 10.7671/j.issn.1001-411X.202306023

    不同良种杜仲叶主要营养成分的动态变化与综合评价

    Dynamic change and comprehensive evaluation of main nutrients in leaves of different Eucommia ulmoides improved varieties

    • 摘要:
      目的 杜仲叶在医疗、保健、食品及无抗养殖等领域有广泛用途。通过比较不同良种杜仲叶主要营养成分的差异及动态变化,筛选优质叶用杜仲良种资源,为杜仲叶的高效利用提供参考。
      方法 分别利用HPLC法和全自动氨基酸分析仪测定13个良种不同生长期杜仲叶9种重要活性成分和15种氨基酸组分的含量,基于主成分分析和隶属函数法开展不同良种杜仲叶的营养评价。
      结果 与槲皮素、桃叶珊瑚苷和京尼平苷酸相比,原儿茶酸、芦丁、绿原酸、紫云英苷、山奈酚以及松脂素二葡萄糖苷的含量在不同良种杜仲叶之间差异较大;不同良种同一活性成分含量的生长动态变化趋势基本一致。13个良种杜仲叶必需氨基酸与总氨基酸的比值(EAA/TAA)为0.40~0.45,必需氨基酸与非必需氨基酸的比值(EAA/NEAA)为0.66~0.82;天冬氨酸、苏氨酸、丝氨酸等5种氨基酸的动态变化趋势较为一致。总氨基酸、必需氨基酸、亮氨酸等9种组分的含量可作为杜仲叶营养价值的评判指标。
      结论 13个良种杜仲叶的氨基酸组成和比例均达到理想蛋白质模式的标准。‘华仲10号’‘华仲24号’‘华仲13号’‘华仲30号’‘华仲12号’是优质的叶用杜仲良种资源,在规模化提取和利用杜仲叶黄酮类时应优先选用‘华仲12号’‘华仲24号’良种。

       

      Abstract:
      Objective Eucommia ulmoides leaves are of wide uses in medical treatment, health care, food industry and antimicrobial-free breeding. To give a reference for the efficient utilization of E. ulmoides leaves, the high grade leaf-used resources were screened after comparing differences and dynamic changes of main nutrients in leaves of different improved varieties.
      Method The contents of nine important active compounds and 15 amino acid components were determined in different leaf growth periods of 13 E. ulmoides improved varieties by HPLC method and automatic amino acid analyzer, respectively. Then the nutritional evaluation of E. ulmoides leaves was performed by principal component analysis and membership function.
      Result Compared to quercetin, aucubin and geniposidic acid, the content differences of protocatechuic acid, rutin, chlorogenic acid, astragalin, kaempferol and abietin pinoresinol diglucoside in E. ulmoides leaves were more significant in the 13 varieties. To content of the same active compound, similar growth dynamic changes were found in different varieties. Among the 13 improved varieties, the ratio of essential amino acids to total amino acids (EAA/TAA) was 0.40−0.45, while the ratio of essential amino acids to non-essential amino acids (EAA/NEAA) was 0.66−0.82. Similar dynamic changes were found in contents of five amino acids including aspartic acid, threonine and serine. The contents of nine components, including total amino acids, essential amino acids and leucine, were selected as indexes for evaluating the nutritional value of the leaves.
      Conclusion The amino acid composition and proportion in leaves of all 13 improved varieties reach the standard of the ideal protein pattern. Varieties of ‘Huazhong 10’ ‘Huazhong 24’ ‘Huazhong 13’ ‘Huazhong 30’ ‘Huazhong 12’ are of high grade improved varieties for E. ulmoides leaf use. ‘Huazhong 12’ and ‘Huazhong 24’ can be preferentially selected while extracting and utilizing leaf flavonoids in large scale.

       

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