Analysis of blast resistance genes and panicle neck blast resistances of conventional indica rice varieties
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摘要:目的
明确常规籼稻品种资源所携带的稻瘟病抗性基因及抗性效应。
方法利用PARMS SNP分型技术，检测14个稻瘟病抗性基因在121份常规籼稻品种中的分布情况，并进行田间穗颈瘟自然鉴定，分析基因型和抗性的关系。
结果大多数供试品种携带2~6个稻瘟病抗性基因，Pi46和Pia的检出率较低，分别为3.3%和7.4%；Pi54和Pi5检出率较高，分别为86.0%和67.8%；所有供试品种均不携带Pi9、Pigm、Pik-m和Pik。田间抗性鉴定结果表明，供试品种的穗颈瘟抗性普遍较弱，但广东品种的穗颈瘟抗性明显好于广西品种的；携带的抗性基因数量与穗颈瘟抗性间相关性不显著；Pi2和Pid3对穗颈瘟抗性贡献显著，优势比值分别为5.98和7.50；Pi2+Pid3+、Pi2+Pi33+和Pid3+Pi33+组合的田间穗颈瘟抗性表现较好。
结论本研究结果为两广籼稻区稻瘟病抗性基因聚合育种的亲本选择提供了理论支持，为常规稻的合理布局提供了科学参考。
Abstract:ObjectiveTo indentify the blast resistance genes and resistance effects of conventional indica rice variety resources.
MethodThe distribution of 14 blast resistance genes in 121 conventional indica rice varieties were genotyped using PARMS SNP typing technology. Natural identification of panicle neck blast was conducted in the field, and the relationship between genotype and resistance was analyzed.
ResultMost of the tested varieties carried 2−6 blast resistance genes. The detection rates of Pi46 and Pia were 3.3% and 7.4%, respectively. The detection rates of Pi54 and Pi5 were 86.0% and 67.8%, respectively. None of the tested varieties carried Pi9, Pigm, Pik-m, or Pik. Field resistance identification showed the resistances to panicle neck blast of the tested varieties were generally weak, yet the resistances of Guangdong varieties were significantly higher than those of Guangxi varieties. There was no significant correlation between the number of resistance genes and the resistance to panicle neck blast. Pi2 and Pid3 had significant contributions on the resistance to panicle neck blast, with the odds ratios of 5.98 and 7.50, respectively. The combinations of Pi2+Pid3+, Pi2+Pi33+ and Pid3+Pi33+ showed higher resistance to panicle neck blast.
ConclusionThe results of this study provides a theoretical support for the parent selection of pyramiding breeding using rice blast resistance genes in the indica rice regions of Guangdong and Guangxi, and provides scientific references for the rational layout of conventional rice.
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Keywords:
- Indica rice /
- Rice blast resistance gene /
- Gene detection /
- Panicle neck blast
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图 1 抗性基因在供试品种中的分布频率
Figure 1. Distribution frequency of resistance genes in tested varieties
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图 2 供试品种携带不同抗性基因数分布
Figure 2. Distribution of resistance gene number carried by tested varieties
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图 3 品种携带抗性基因数量与抗级间的关系分析
Figure 3. Analysis of the resistance gene number carried by each variety and resistance level
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图 4 单个抗性基因对穗颈瘟抗性贡献的逻辑回归分析(a)及平均抗级(b)
图a中黑点上下黑线分别为各优势比的95%置信区间；图b中绿色柱表示携带某基因的平均抗级，蓝色柱表示不携带某基因的平均抗级，“**”表示与不携带该基因的材料差异显著(P<0.01， t检验)
Figure 4. Logistic regression analysis of contribution to resistance (a) and average resistance (b) of single gene to panicle neck blast
In figure a, the black lines above and below each black dot represent the 95% confidence intervals for each odds ratio; In figure b, the green column represents the average resistance of carrying a certain gene, the blue column represents the average resistance level of not carrying a certain gene , and “**” indicates significant difference from the material not carrying the gene (P<0.01, t test)
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图 5 不同稻瘟病抗性基因组合的抗级差异
Figure 5. Resistance level of different rice blast gene combinations
表 1 不同类型供试品种间抗性基因分布频率
Table 1 Distribution frequency of resistance genes in different types of tested varieties
% 品种类型
Type of varietyPi2 Pi5 Pi33 Pi46 Pi54 Pib Pid3 Pit Pita Pia 广西品种
Guangxi varieties13.3 74.7 22.7 5.3 81.3 33.3 10.7 28.0 44.0 5.3 广东品种
Guangdong varieties60.9 56.5 10.9 0 84.3 43.5 50.0 21.7 47.8 10.9 2011年前广西品种
Guangxi varieties before 20110 61.3 25.8 12.9 80.6 32.3 0 16.1 51.6 6.5 2011年前广东品种
Guangdong varieties before 201142.3 69.2 3.8 0.0 92.3 34.6 34.6 23.1 57.7 7.7 2011年后广西品种
Guangxi varieties after 201122.7 84.1 20.5 0.0 81.8 34.1 18.2 36.4 36.4 4.5 2011年后广东品种
Guangdong varieties after 201185.0 40.0 20.0 0.0 95.0 55.0 70.0 20.0 35.0 15.0 
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表 2 供试品种穗颈瘟抗级分布频率
Table 2 Distribution frequency of resistance level to panicle neck blast in tested varieties
% 品种类型
Type of variety0
(高抗 Highly resistant)1
(抗 Resistant)3
(中抗 Medium resistrant)5
(中感 Medium susceptible)7
(感 Susceptible)9
(高感 Highly susceptible)总供试品种
All tested vararieties0 4.9 15.7 28.1 18.2 33.1 广西品种
Guangxi varieties0 0 10.7 25.3 18.7 45.3 广东品种
Guangdong varieties0 13.1 23.9 32.6 17.4 13.0
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