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    隋承融, 李绮琪, 潘铃慧, 等. 禁食对黄羽肉鸡肠道屏障和感觉迷走神经受体表达的影响[J]. 华南农业大学学报, 2024, 45(2): 172-178. doi: 10.7671/j.issn.1001-411X.202211048
    引用本文: 隋承融, 李绮琪, 潘铃慧, 等. 禁食对黄羽肉鸡肠道屏障和感觉迷走神经受体表达的影响[J]. 华南农业大学学报, 2024, 45(2): 172-178. doi: 10.7671/j.issn.1001-411X.202211048
    shu
    SUI Chengrong, LI Qiqi, PAN Linghui, et al. Effects of fasting on intestinal barrier and sensory vagus receptor expression in yellow-feathered broilers[J]. Journal of South China Agricultural University, 2024, 45(2): 172-178. doi: 10.7671/j.issn.1001-411X.202211048
    Citation: SUI Chengrong, LI Qiqi, PAN Linghui, et al. Effects of fasting on intestinal barrier and sensory vagus receptor expression in yellow-feathered broilers[J]. Journal of South China Agricultural University, 2024, 45(2): 172-178. doi: 10.7671/j.issn.1001-411X.202211048
    shu

    禁食对黄羽肉鸡肠道屏障和感觉迷走神经受体表达的影响

    Effects of fasting on intestinal barrier and sensory vagus receptor expression in yellow-feathered broilers

      摘要
    • 摘要:
      目的 探究饥饿状态下黄羽肉鸡肠道炎症信号以及迷走感觉神经相关受体表达的变化。
      方法 试验选用黄羽肉鸡，根据体质量一致原则随机分为对照组和禁食组。样品通过q-PCR检测黄羽肉鸡下丘脑食欲肽、肠道紧密连接蛋白和炎症因子以及结状神经节(Nodose ganglia，NG)内炎症和食欲调控相关受体的表达；利用电镜扫描和HE染色观察并统计肠道形态变化。
      结果 与对照组相比，黄羽肉鸡短期禁食可提高下丘脑促采食食欲肽AgRP(P<0.05)和NPY(P<0.01)表达；短期禁食升高肠道H2O2浓度，促进小肠紧密连接蛋白表达(P<0.05)，降低空肠损伤比例(P<0.01)，增加空肠绒毛吸收能力(P<0.01)；短期禁食增加空肠黏膜抗炎因子IL-4和IL-10表达(P<0.01)；此外，短期禁食不仅促进NG内食欲调控相关受体FFAR2和NPY2R表达(P<0.01)，而且提高抗炎因子受体IL-4R表达(P<0.01)。
      结论 饥饿可提高黄羽肉鸡肠道抗炎因子水平，增强肠道屏障功能，并促进迷走感觉神经抗炎因子等受体表达。

       

      Abstract:
      Objective  The aim of this study was to investigate the changes of intestinal inflammatory signals and vagal nerve related receptors in yellow-feathered broilers under starvation.
      Method Yellow-feathered broilers were used in the experiment. They were randomly divided into control group and fasting group according to the principle of consistent body weight. The expressions of appetite peptides, intestinal tight junction proteins and inflammatory factors in hypothalamus and receptors related to inflammation and appetite regulation in nodose ganglia (NG) of yellow-feathered broilers were detected by q-PCR. The morphological changes of intestinal tract were observed and statistically analyzed by electron microscopy and HE staining.
      Result Compared with the control group, short-term fasting increased the expressions of hypothalamic orexigenic peptides AgRP (P<0.05) and NPY (P<0.01). Short-term fasting increased the concentration of intestinal H2O2, promoted the expressions of small intestinal tight junction protein (P<0.05), decreased the proportion of jejunal injury (P<0.01), and increased the absorption capacity of jejunal villi (P<0.01). Short-term fasting increased the expressions of anti-inflammatory factors IL-4 and IL-10 in jejunal mucosa (P<0.01). In addition, short-term fasting not only promoted the expressions of FFAR2 and NPY2R in NG (P<0.01), but also increased the expression of IL-4R (P<0.01).
      Conclusion Starvation can increase the levels of intestinal anti-inflammatory factors, enhance intestinal barrier function, and promote the expressions of receptors such as vagal anti-inflammatory factors in yellow-feathered broilers.

       

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