饶雪琴, 唐瑞, 李华平. 香蕉枯萎病菌1号和4号生理小种在‘巴西蕉’植株及根际土壤中的时空分布[J]. 华南农业大学学报, 2023, 44(4): 563-569. DOI: 10.7671/j.issn.1001-411X.202210014
    引用本文: 饶雪琴, 唐瑞, 李华平. 香蕉枯萎病菌1号和4号生理小种在‘巴西蕉’植株及根际土壤中的时空分布[J]. 华南农业大学学报, 2023, 44(4): 563-569. DOI: 10.7671/j.issn.1001-411X.202210014
    RAO Xueqin, TANG Rui, LI Huaping. Spatiotemporal distribution of Fusarium oxysporum f. sp. cubense race 1 and race 4 in Brazilian plant and rhizosphere soil[J]. Journal of South China Agricultural University, 2023, 44(4): 563-569. DOI: 10.7671/j.issn.1001-411X.202210014
    Citation: RAO Xueqin, TANG Rui, LI Huaping. Spatiotemporal distribution of Fusarium oxysporum f. sp. cubense race 1 and race 4 in Brazilian plant and rhizosphere soil[J]. Journal of South China Agricultural University, 2023, 44(4): 563-569. DOI: 10.7671/j.issn.1001-411X.202210014

    香蕉枯萎病菌1号和4号生理小种在‘巴西蕉’植株及根际土壤中的时空分布

    Spatiotemporal distribution of Fusarium oxysporum f. sp. cubense race 1 and race 4 in Brazilian plant and rhizosphere soil

    • 摘要:
      目的 香蕉Musa spp.是我国重要的经济作物之一,由尖孢镰刀菌古巴专化型Fusarium oxysporum f. sp. cubense (Foc) 引起的香蕉枯萎病蔓延快、根治难,严重阻碍了我国香蕉产业发展。由于Foc菌量和空间分布与其侵染和致病作用直接相关,探索Foc在‘巴西蕉’Musa acuminate L. AAA group, cv. Brazilian植株和土壤中的时空分布具有重要意义。
      方法 采用伤根接种法对‘巴西蕉’苗分别接种香蕉枯萎病菌1号生理小种(Foc1)和4号生理小种(Foc4),通过实时荧光定量PCR技术检测接种后不同时间、不同香蕉组织及根际土壤中的菌量,分析了香蕉枯萎病菌在植株和土壤中的时空分布。
      结果 温室中接种Foc后2~21 d内,‘巴西蕉’根部和球茎中Foc1和Foc4菌量随时间延长而逐渐增加,但Foc4菌量始终大于Foc1;在‘巴西蕉’球茎中,Foc1和Foc4分别在接种后21和14 d菌量达到最大。田间接种Foc后30 d,离‘巴西蕉’植株地面半径(r)5 cm、地下深度25~30 cm土壤中的Foc1和Foc4菌量最大;而r为15和30 cm时,地下深度10~15 cm土壤中的Foc菌量大于0~5和25~30 cm的菌量;在相同r和地下深度的‘巴西蕉’根际土壤中,一般Foc4菌量大于Foc1。
      结论 Foc1和Foc4菌量在‘巴西蕉’植株和根际土壤中具有明显不同的时空分布,同一空间中Foc4菌量大于Foc1菌量,研究结果可为香蕉枯萎病的发生流行、预测预报和防控提供一定的理论指导。

       

      Abstract:
      Objective Banana (Musa spp.) is one of the important economic crops in China. However, banana fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) seriously devastates the development of banana industry in China due to the rapid spread and difficult control of this disease. It is important to explore the spatial and temporal distribution of Foc in Brazilian (Musa acuminate L. AAA group, cv. Brazilian) plant and rhizosphere soil, which are directly related to infection and pathogenicity of Foc.
      Method Brazilian seedlings were inoculated with race 1 (Foc1) or race 4 (Foc4) by wounding roots in greenhouse, the amount and spatial distribution of Foc in different tissues at different times and in different rhizosphere soil of banana seedlings were analyzed using real-time quantitative PCR.
      Result From 2 to 21 days after inoculation, the contents of Foc1 and Foc4 in Brazilian roots and rhizomes increased with time, and the contents of Foc4 were significantly higher than those of Foc1. The contents of Foc1 and Foc4 in Brazilian rhizomes reached the highest at 21 and 14 days after inoculation, respectively. In the field, Foc1 and Foc4 were the most abundant in soil at the ground radius (r) of 5 cm from Brazilian plant and the depth of 25 to 30 cm under the ground at 30 days after inoculation. When the r values were 15 and 30 cm, the contents of Foc in soil at the depth of 10~15 cm were higher than those of 0~5 cm and 25~30 cm. In Brazilian rhizosphere soil at the same r value and soil depth, the contents of Foc4 were higher than those of Foc1 in most cases.
      Conclusion Foc1 and Foc4 have a significantly different spatial and temporal distribution in Brazilian plant and rhizosphere soil, and the content of Foc4 is higher than that of Foc1 in the same space, which provides a theoretical guidance for the epidemic, prediction and prevention and control of banana fusarium wilt.

       

    /

    返回文章
    返回