Research status and prospect on bacterial 3-oxoacyl-ACP-reductase
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摘要:
3–酮脂酰ACP还原酶属于短链醇脱氢酶/还原酶(SDR)超家族蛋白，参与细菌脂肪酸及相关物质合成，负责催化3–酮脂酰ACP还原为3–羟脂酰ACP。3–酮脂酰ACP还原酶在细菌中广泛存在，且氨基酸序列较为保守，然而其生物学功能却展现出多样性。本文对近年来细菌3–酮脂酰ACP还原酶的结构特性、生物学功能和抑制剂等方面的研究进展进行综述，为加深对3–酮脂酰ACP还原酶的理解和抗菌药物的开发提供有益的借鉴。
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关键词:
- 3–酮脂酰ACP还原酶 /
- II型脂肪酸合成系统 /
- 抑制剂
Abstract:3-oxoacyl-ACP-reductase, a member of short chain dehydrogenase/reductase(SDR) super family, participates in the biothsythesis of bacterial fatty acid and derivatives by catalyzing the reduction of 3-oxoacyl-ACP to 3-hydroxyacyl-ACP. 3-oxoacyl-ACP-reductase is ubiquitously exsit in bacterial and highly conversed in amino acid sequence, however, it has diverse biological functions. In this review, we summerize the advance of structures, biological functions and inhibitors of 3-oxoacyl-ACP-reductase in recent years. This paper will provide useful references for further understanding of 3-oxoacyl-ACP-reductase and antibacterial drug design.
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Keywords:
- 3-oxoacyl-ACP-reductase /
- Fatty acid synthesis system II /
- Inhibitor
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图 1 细菌脂肪酸合成途径(A)与OAR催化反应机制(B)
Figure 1. The pathway of fatty acid biosynthesis in bacterial (A) and the reduction of 3-oxoacyl-ACP catalyzed by 3-oxoacyl-ACP reductase (B)
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图 2 不同细菌中的脂肪酸合成基因簇与OAR编码基因
Figure 2. The fatty acid biosynthesis gene cluster and gene enconding 3-oxoacyl-ACP reductase in different bacteria
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图 3 大肠埃希菌FabG的单体(A)和四聚体(B)晶体结构
Figure 3. The crystal structures of Escherichia coli FabG monomer (A) and tetramer (B)
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图 4 大肠埃希菌FabG假定催化机制(A)与突变体蛋白重建脂肪酸合成反应(B)
Figure 4. The postulated catalytic mechanism of Escherichia coli FabG (A) and reconstruction of fatty acid by mutant protein (B)
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图 5 苜蓿中华根瘤菌NodG具有OAR活性并参与苜蓿结瘤
A：苜蓿根瘤菌FabG与NodG具有OAR活性；1：C8:0-ACP，2：EcFabG，3：苜蓿根瘤菌FabG，4：苜蓿根瘤菌NodG，5：不添加FabG蛋白，6：C8:0-ACP。B：NodG相关菌株结瘤效率；S. meliloti 1021/pSRK-Gm：野生型菌株携带空质粒，LF1：苜蓿根瘤菌nodG突变菌株，LF2：苜蓿根瘤菌nodG/fabG双突变菌株携带苜蓿根瘤菌nodG质粒，LF3：苜蓿根瘤菌nodG/fabG双突变菌株携带苜蓿根瘤菌fabG质粒， “*”“**”分别表示0.05和0.01水平差异显著[43]，每个试验有8个重复
Figure 5. The Sinorhizobium meliloti NodG maintains OAR activity and involves in alfalfa nodulation
A: Sinorhizobium meliloti FabG and NodG maintain the OAR activity; 1: C8:0-ACP, 2: Product of EcFabG, 3: Product of SmFabG, 4: Product of SmNodG, 5: No FabG addition, 6: C8:0-ACP. B: Nodulation efficiency of Sinorhizobium meliloti mutant strains; S. meliloti 1021/pSRK-Gm: Wild type strain carrying plasmid pSRK-Gm, LF1: Sinorhizobium meliloti nodG mutant strain, LF2: Sinorhizobium meliloti nodG/fabG double mutant strain carrying SmnodG ecoding plasmid, LF3: Sinorhizobium meliloti nodG/fabG double mutant strain carrying SmfabG ecoding plasmid, “*” and “**”indicate significant differences at levels of 0.05 and 0.01, respectively, Every experiment has eight replicates
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图 6 XccfabG2互补OAR突变菌株及参与Xcc DSF类信号分子合成
A：XccfabG2互补大肠埃希菌fabG温度敏感突变菌株CL104；EcfabG：质粒携带有大肠埃希菌fabG基因，Vector：空质粒载体，fabG2：质粒携带有XccfabG2基因，AasS：质粒携带有哈氏弧菌aasS基因，AasS+ fabG：质粒携带有哈氏弧菌aasS和XccfabG2基因。B：XccfabG2互补XccfabG1突变菌株；WT：Xcc野生型，∆fabG1+pfabG2：XccfabG1突变菌株携带有XccfabG2表达质粒。C: XccfabG2突变菌株的DSF类信号分子产量；DSF：11−甲基−顺−2−月桂酰烯酸，BDSF：顺−2−月桂酰烯酸，空柱：Xcc野生型，黑柱：XccfabG2突变菌株，灰柱：XccfabG2突变菌株携带有XccfabG2表达质粒，条状柱：XccfabG2突变菌株携带有XccfabG1表达质粒
Figure 6. XccfabG2 complements OAR mutant strain and invovles in DSF signal synthesis in Xcc
A: XccfabG2 complement E. coli fabG temperature sensitive strain CL104; EcfabG: CL104 carrying plasmid expressing EcfabG, Vector: CL104 carrying empty plasmid, fabG2: CL104 carrying plasmid expressing XccfabG2, AasS: CL104 carrying plasmid expressing Vibrio harzii aasS, AasS+ fabG2: CL104 carrying plasmid expressing Vibrio harzii aasS and XccfabG2. B: XccfabG2 complement XccfabG1 mutant strain; WT: Wild type X. campestris pv. campestris strain, ∆fabG1+pfabG2: XccfabG1 mutant strain carrying XccfabG2 expressing plasmid. C: The DSFs production of XccfabG2 mutant strains; DSF: cis-11-methyl-2-dodecenoic acid, BDSF: cis-2-dodecenoic acid, White column: Wild-type X. campestris pv. campestris strain, Black column: XccfabG2 mutant strain, Gray column: XccfabG2 mutant strain carrying a plasmid encoding XccfabG2, Stippled column: XccfabG2 mutant strain carrying a plasmid encoding XccfabG1
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图 7 XccfabG3互补CL104并参与菌黄素合成
A：XccfabG3互补大肠埃希菌fabG温度敏感突变菌株CL104；EcfabG:质粒携带有大肠埃希菌fabG基因，fabG1：质粒携带有XccfabG1基因，Vector：空质粒载体，fabG3：质粒携带有XccfabG3基因，CL104：无外源质粒。B： XccfabG3突变菌株的菌黄素产量；Xcc WT：Xcc野生型，Xcc YH1：XccfabG3突变菌株，Xcc YH2：XccfabG3突变菌株携带有XccfabG3表达质粒，Xcc YH6：XccfabG3突变菌株携带有XccfabG1表达质粒，Xcc YH10：XccfabG3突变菌株携带有XccfabG2表达质粒，Xcc YH7：XccfabG3突变菌株携带有EcfabG表达质粒，Xcc YH1+3-HBA：XccfabG3突变菌株添加3−羟基丁酸
Figure 7. XccfabG3 complements CL104 and invovles in xanthomonadin synthesis in Xcc
A: XccfabG3 complement Escherichia coli fabG temperature sensitive strain CL104; EcfabG: CL104 carrying plasmid expressing EcfabG, fabG1: CL104 carrying plasmid expressing XccfabG1, Vector: CL104 carrying empty plasmid, fabG3: CL104 carrying plasmid expressing XccfabG3, CL104: CL104 without plasmid. B: Xanthomonadin production in XccfabG3 mutant; Xcc WT: Wild-type X. campestris pv. campestris strain, Xcc YH1: XccfabG3 mutant, Xcc YH2: XccfabG3 mutant carrying XccfabG3 ecoding plasmid, Xcc YH6: XccfabG3 mutant carrying XccfabG1 ecoding plasmid, Xcc YH10: XccfabG3 mutant carrying XccfabG2 ecoding plasmid, Xcc YH7: XccfabG3 mutant carrying EcfabG ecoding plasmid, Xcc YH1+3-HBA: XccfabG3 mutant supplemented with 3-hydroxybutyric acid
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