Abstract: Deoxynivalenol (DON) is one of the most common and serious polluted mycotoxins that contaminate grains, feed ingredients and feed. The acute or chronic poisoning symptoms of feed-borne exposure to DON in animals are vomiting, diarrhea, feed refusal, weight loss and even death, which seriously threatens the healthy breeding of animal. The toxicity mechanism and metabolic transformation of DON are the research hotspots in the fields of agriculture and food. This article reviews the latest domestic and international research progress in the cytotoxicological mechanism, biological prevention methods and detoxification microorganism screening of DON. It is expected to provide references for prevention and control of the harm of DON to animal.