MicroRNA-1285及其靶标DDX3X对猪塞内卡病毒感染PK-15细胞的调控作用

孙媛; 唐晓钰; 白杨; 陈雨琪; 郑瑶瑶; 吴佼玲; 蓝天; 马静云

doi:10.7671/j.issn.1001-411X.202205038

MicroRNA-1285及其靶标DDX3X对猪塞内卡病毒感染PK-15细胞的调控作用

Regulation effects of microRNA-1285 and its target DDX3X on Senecavirus A infected PK-15 cells

摘要

摘要:
目的探究MicroRNA-1285(miR-1285)及其靶标DDX3X在猪塞内卡病毒(Senecavirus A，SVA)感染PK-15细胞中的调控作用。

方法利用qRT-PCR、双荧光素酶活性及Western blot等方法研究miR-1285和DDX3X对I型干扰素(IFN-β)分泌及RIG-I信号通路的作用，分析miR-1285及DDX3X对SVA 3C蛋白基因表达的影响。

结果 SVA感染PK-15细胞后，miR-1285表达量显著升高，并且miR-1285与DDX3X存在负靶向关系，二者可促进IFN-β转录及蛋白水平的表达。miR-1285通过靶向DDX3X对RIG-I信号通中的MAVS、TRAF3信号分子起调控作用。对于SVA 3C蛋白基因，DDX3X可以显著抑制其转录，并且可以逆转miR-1285所诱导的上调趋势。

结论 SVA感染PK-15细胞后，宿主miR-1285及其靶标DDX3X对IFN-β及病毒3C蛋白的表达具有调控作用，研究结果将为明确miRNAs调控SVA感染的分子机制奠定基础，并为SVA的防控和诊断提供新的科学依据。

Abstract:
Objective To explore the regulation roles of microRNA-1285 (miR-1285) and its target DDX3X in Senecavirus A (SVA) infected PK-15 cells.

Method By qRT-PCR, double luciferase activity and Western blot, the effects of miR-1285 and its target DDX3X on IFN-β secretion and the RIG-I signaling pathway were studied, and their effects on the expression of SVA 3C protein gene were analyzed.

Result In SVA infected PK-15 cells, the expression of miR-1285 increased significantly, and there was a negative targeting relationship between miR-1285 and DDX3X. Both miR-1285 and DDX3X promoted the transcription and protein expression of IFN-β. MiR-1285 regulated MAVS and TRAF3 signaling molecules in the RIG-I signaling pathway by targeting DDX3X. For SVA 3C protein, DDX3X significantly inhibited the transcription of 3C and reversed the up-regulation trend induced by miR-1285.

Conclusion After infecting PK-15 cells with SVA, host miR-1285 and its target DDX3X can regulate the expression of IFN-β and the viral 3C protein, which will lay a foundation for clarifying the molecular mechanism of miRNAs regulating SVA infection, and provide a new scientific basis for the prevention, control and diagnosis of SVA.

HTML全文

参考文献(54)

施引文献

资源附件(0)