周志成, 孙海, 肖仲久, 等. 辣椒炭疽病病原分离鉴定及对杀菌剂敏感性测定[J]. 华南农业大学学报, 2023, 44(3): 430-437. DOI: 10.7671/j.issn.1001-411X.202203058
    引用本文: 周志成, 孙海, 肖仲久, 等. 辣椒炭疽病病原分离鉴定及对杀菌剂敏感性测定[J]. 华南农业大学学报, 2023, 44(3): 430-437. DOI: 10.7671/j.issn.1001-411X.202203058
    ZHOU Zhicheng, SUN Hai, XIAO Zhongjiu, et al. Identification and fungicide sensitivity of pathogen causing anthracnose of pepper[J]. Journal of South China Agricultural University, 2023, 44(3): 430-437. DOI: 10.7671/j.issn.1001-411X.202203058
    Citation: ZHOU Zhicheng, SUN Hai, XIAO Zhongjiu, et al. Identification and fungicide sensitivity of pathogen causing anthracnose of pepper[J]. Journal of South China Agricultural University, 2023, 44(3): 430-437. DOI: 10.7671/j.issn.1001-411X.202203058

    辣椒炭疽病病原分离鉴定及对杀菌剂敏感性测定

    Identification and fungicide sensitivity of pathogen causing anthracnose of pepper

    • 摘要:
      目的  明确辣椒炭疽病病原菌Colletotrichum spp.种类,并筛选防治药剂。
      方法  采集贵州省贵阳市花溪区具有典型炭疽病症状的‘党武’辣椒叶片和果实,分离病原菌;采用单孢分离法、柯赫氏法则、形态学特征结合ITS、GADPH、CHS-1ACT多基因序列分析,明确病原菌的致病能力及分类地位;采用菌丝生长速率法测定病原菌对6种化学杀菌剂和6种生物杀菌剂的敏感性。
      结果  病原菌鉴定为斯高维尔炭疽菌Colletotrichum scovillei;室内药剂敏感性测定发现12种杀菌剂对C. scovillei均有一定的抑制作用,75%(w)肟菌·戊唑醇WDG、10%(w)苯醚甲环唑WDG和250 g/L吡唑醚菌酯SC的抑制效果最好,EC50分别为0.254、0.731、0.745 mg/L。其次是200 g/L异硫氰酸烯丙酯SL、3%(w)中生菌素WP、10 g/L申嗪霉素SC、80 g/L宁南霉素AS、200 g/L异硫氰酸烯丙酯EW和10 g/L蛇床子素ME,EC50分别为1.238、1.307、1.711、2.929、3.175和2.191 mg/L。将10%(w)苯醚甲环唑WDG和250 g/L吡唑醚菌酯SC按照1∶4、2∶3的体积比复配时,联合毒力测定和评价结果显示两者复配对该病菌具有协同增效作用。
      结论  本研究可为辣椒生产中辣椒炭疽病的田间药剂防治提供参考。

       

      Abstract:
      Objective  To identify the pathogenic fungi of pepper anthracnose and screen the fungicides with strong inhibitory effects.
      Method  The typical leaves and fruits with anthracnose symptoms were collected from the peppers named ‘Dangwu’ in Huaxi, Guiyang, Guizhou. The pathogen was isolated. Its pathogenicity and classification were determined by monospore isolation, Koch’s postulates, and morphological characteristics combined with multi-locus (ITS, ACT, GAPDH andCHS-1) phylogenetic analysis. The sensitivities of pathogen to six chemical fungicides and six biological fungicides were investigated by the mycelial growth rate method.
      Result  The pathogen causing pepper ‘Dangwu’ anthracnose in Huaxi was identified as Colletotrichum scovillei. The indoor fungicide sensitivity test showed that 12 fungicides all had certain inhibitory effect against C. scovillei. The inhibition effects of 75%(w) trifloxystrobin·tebuconazole WDG, 10%(w) difenoconazole WDG and 250 g/L pyraclostrobin SC were higher with the EC50 of 0.254, 0.731 and 0.745 mg/L, respectively, followed by 200 g/L allylisothiocyanate SL, 3% (w) zhongshengmycin WP, 10 g/L phenazine-1-carboxylic acid SC, 80 g/L ningnanmycin AS, 200 g/L allylisothiocyanate EW and 10 g/L osthol ME with the EC50 of 1.238, 1.307, 1.711, 2.929, 3.175 and 2.191 mg/L, respectively. 10% (w) difenoconazole WDG and 250 g/L pyraclostrobin SC mixed at the volume ratio of 1∶4 or 2∶3 showed an obvious synergistic effect on inhibiting the pathogen based on the joint toxicity test and evaluation results.
      Conclusion  This study can provide references for the field prevention and control of pepper anthracnose using pesticides.

       

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