高级检索
    陈林, 赵伟杰, 张枫琳, 等. 不同共轭亚油酸异构体对小鼠脂肪沉积、能量代谢和肠道微生物的影响[J]. 华南农业大学学报, 2022, 43(3): 1-8. DOI: 10.7671/j.issn.1001-411X.202110016
    引用本文: 陈林, 赵伟杰, 张枫琳, 等. 不同共轭亚油酸异构体对小鼠脂肪沉积、能量代谢和肠道微生物的影响[J]. 华南农业大学学报, 2022, 43(3): 1-8. DOI: 10.7671/j.issn.1001-411X.202110016
    shu
    CHEN Lin, ZHAO Weijie, ZHANG Fenglin, et al. Effects of different conjugated linoleic acid isomers on fat deposition, energy metabolism and gut microbiota in mice[J]. Journal of South China Agricultural University, 2022, 43(3): 1-8. DOI: 10.7671/j.issn.1001-411X.202110016
    Citation: CHEN Lin, ZHAO Weijie, ZHANG Fenglin, et al. Effects of different conjugated linoleic acid isomers on fat deposition, energy metabolism and gut microbiota in mice[J]. Journal of South China Agricultural University, 2022, 43(3): 1-8. DOI: 10.7671/j.issn.1001-411X.202110016
    shu

    不同共轭亚油酸异构体对小鼠脂肪沉积、能量代谢和肠道微生物的影响

    Effects of different conjugated linoleic acid isomers on fat deposition, energy metabolism and gut microbiota in mice

      摘要
    • 摘要:
      目的  探究日粮添加不同共轭亚油酸(Conjugated linoleic acid，CLA)异构体对小鼠体脂、能量代谢和肠道微生物的影响。
      方法  用普通日粮(对照组)、普通日粮分别添加质量分数为2%的顺9，反11−共轭亚油酸(c9, t11-CLA)和反10，顺12−共轭亚油酸(t10, c12-CLA)饲喂小鼠12周，检测小鼠脂肪含量、能量代谢和肠道微生物的变化。
      结果  与对照组相比，c9, t11-CLA和t10, c12-CLA组小鼠体质量分别降低了22.79%和25.04%；脂肪含量分别降低了57.98%和59.41%；小鼠皮下脂肪、附睾白色脂肪以及褐色脂肪含量也显著降低。c9, t11-CLA和t10, c12-CLA均显著提高小鼠耗氧量和产热量，其中，t10, c12-CLA显著降低小鼠夜间的呼吸交换率。16S rRNA测序结果显示，3组小鼠的肠道微生物菌群结构存在明显差异，c9, t11-CLA和t10, c12-CLA组小鼠肠道中与机体代谢相关的菌群丰度显著高于对照组，且t10, c12-CLA能促进与脂质代谢相关的菌群丰度上调。在门水平，c9, t11-CLA和t10, c12-CLA组厚壁菌门和放线菌门相对丰度显著上调，拟杆菌门和疣微菌门相对丰度显著下调；在属水平，与对照组相比，c9, t11-CLA组双歧杆菌属相对丰度显著上调，Ileibacterium和Akkermansia相对丰度显著下调。
      结论  日粮添加c9, t11-CLA或t10, c12-CLA，可显著降低小鼠脂肪沉积，这可能与CLA促进机体能量代谢和改变肠道微生物菌群结构有关。

       

      Abstract:
      Objective  The purpose of this study was to investigate the effects of dietary supplementation of different conjugated linoleic acid (CLA) isomers on body fat, energy metabolism and gut microbiota in mice.
      Method  Mice were fed with normal diet (control group), normal diet supplemented with 2% cis-9, trans-11 conjugated linoleic acid (c9, t11-CLA) and trans-10, cis-12 conjugated linoleic acid (t10, c12-CLA) for 12 weeks respectively. The changes of fat content, energy metabolism and gut microbiota were detected.
      Result  Compared with the control group, the body weight of mice in the c9, t11-CLA and t10, c12-CLA groups decreased by 22.79% and 25.04% respectively, the fat contents decreased by 57.98% and 59.41% respectively, and the contents of subcutaneous fat, epididymal white fat and brown fat also decreased significantly. In addition, c9, t11-CLA and t10, c12-CLA significantly increased oxygen consumption and heat production in mice, and t10, c12-CLA significantly decreased the nocturnal respiratory exchange rate. The 16S rRNA sequencing results showed that there were obvious differences in gut microbial community structure among three groups of mice. The abundances of metabolism-related flora in the guts of mice in c9, t11-CLA and t10, c12-CLA groups were significantly higher than that in the control group, and t10, c12-CLA could promote the up-regulation of lipid metabolism-related flora. At the phylum level, compared with the control group, the relative abundances of Firmicutes and Actinobacteria in c9, t11-CLA and t10, c12-CLA groups were significantly up-regulated, while the relative abundances of Bacteroidetes and Verrucomicrobia were significantly down-regulated. At the genus level, compared with the control group, the relative abundance of Bifidobacterium in c9, t11-CLA group was significantly up-regulated, while the relative abundances of Ileibacterium and Akkermansia were significantly down-regulated.
      Conclusion  Dietary supplementation with c9, t11-CLA or t10, c12-CLA can significantly reduce fat deposition in mice, which may be related to the elevated energy metabolism and altered gut microbial community structure induced by CLA.

       

      • HTML全文
      • 参考文献(32)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回