马涛, 杨兴翠, 林娜, 等. 梨小食心虫性信息素微胶囊的制备、缓释及野外迷向效果[J]. 华南农业大学学报, 2022, 43(2): 77-86. DOI: 10.7671/j.issn.1001-411X.202103009
    引用本文: 马涛, 杨兴翠, 林娜, 等. 梨小食心虫性信息素微胶囊的制备、缓释及野外迷向效果[J]. 华南农业大学学报, 2022, 43(2): 77-86. DOI: 10.7671/j.issn.1001-411X.202103009
    MA Tao, YANG Xingcui, LIN Na, et al. Preparation, sustained release and field mating disruption of Grapholitha molesta sex pheromone microcapsule[J]. Journal of South China Agricultural University, 2022, 43(2): 77-86. DOI: 10.7671/j.issn.1001-411X.202103009
    Citation: MA Tao, YANG Xingcui, LIN Na, et al. Preparation, sustained release and field mating disruption of Grapholitha molesta sex pheromone microcapsule[J]. Journal of South China Agricultural University, 2022, 43(2): 77-86. DOI: 10.7671/j.issn.1001-411X.202103009

    梨小食心虫性信息素微胶囊的制备、缓释及野外迷向效果

    Preparation, sustained release and field mating disruption of Grapholitha molesta sex pheromone microcapsule

    • 摘要:
      目的  探索梨小食心虫Grapholitha molesta性信息素微胶囊最佳制备方法,并在室内条件下对微胶囊的缓释作用进行评估。
      方法  采用梨小食心虫性信息素(反−8−十二碳烯基乙酸酯)作为芯材,辛烯基琥珀酸淀粉钠、乳化变性淀粉−809、麦芽糊精、β−环糊精和明胶作为壁材,制备梨小食心虫性信息素微胶囊乳液,研究芯材和壁材不同比例等条件对微胶囊乳液粒径分布及包封率的影响,并进行室内缓释和野外迷向试验研究。
      结果  制备微胶囊乳液的最佳壁材配方(质量比)为:辛烯基琥珀酸淀粉钠∶乳化变性淀粉−809∶明胶∶麦芽糊精∶β−环糊精=5∶15∶0∶3∶2,最佳制备条件为:壁材与芯材质量比10∶1,剪切速度10 000 r/min,剪切时间2 min,高压均质压力20 MPa,均质时间2 min;二次均质加入10 g液体石蜡,均质压力20 MPa,均质时间4 min。在30、40和50 ℃条件下进行室内缓释试验,91 d时仍可检测到性信息素残留,而室温下未微胶囊化的性信息素稀释液5 h后完全释放。野外迷向试验中,微胶囊乳液药效可以持续约80 d,用药量(性信息素)为45、75、120和75 g/hm2(冷藏1年)的梨小食心虫性信息素微胶囊乳液的迷向效果无显著差异。
      结论  梨小食心虫性信息素微胶囊乳液显著延长了持效期,可有效用于梨小食心虫的大面积迷向防治。

       

      Abstract:
      Objective  The preparation ofGrapholitha molesta sex pheromone microcapsule was investigated, and the sustained release effect of the microcapsule under indoor conditions was evaluated.
      Method  Adopting G. molesta sex pheromone (E8-dodecenyl acetate) as core material, octenylsuccinate starch sodium, emulsifying starch-809, maltodextrin, ß-cyclodextrin and gelatin as wall materials, G. molesta sex pheromone microcapsule emulsion was prepared. The factors that affect the particle size distribution and encapsulation rate of the microcapsule emulsion, including the different proportions of the core material and the wall material, the sustained release ratio under indoor conditions and the mating disruption under field conditions were studied.
      Result  The optimum wall material formula (mass ratio) of microcapsule emulsion was octenylsuccinate starch sodium∶ emulsifyingstarch-809∶gelatin∶maltodextrin∶β-cyclodextrin=5∶15∶0∶3∶2. The optimum preparation conditions were as follows: The mass ratio of wall material and core material was 10∶1, the shear velocity was 10 000 r/min, the shearing time was 2 min, the high pressure homogenization pressure was 20 MPa, the homogeneous time was 2 min; During the second homogeneous, 10 g liquid paraffin was added, the homogenization pressure was 20 MPa and the second homogeneous time was 4 min. The indoor sustained release experiments were conducted under 30, 40 and 50 ℃. The sex pheromones released from the microcapsules could be detected on the 91st day, whereas the unencapsulated sex pheromone was no longer detectable after 5 h. Field mating disruption study showed that microcapsule emulsion efficacy could last for 80 d and the effects of four sex pheromone dosages of 45, 75, 120 g/hm2 and 75 g/hm2 (refrigerating for one year) had no significant difference.
      Conclusion  The G. molesta sex pheromones microcapsule emulsion significantly prolongs the duration period, and can be applied for the large-scale mating disruption control of G. molesta.

       

    /

    返回文章
    返回