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    吴坤钟, 李荣松, 曹阳, 等. 英诺克李斯特菌侵染果蝇S2细胞后的转录组分析[J]. 华南农业大学学报, 2020, 41(5): 17-26. DOI: 10.7671/j.issn.1001-411X.202001023
    引用本文: 吴坤钟, 李荣松, 曹阳, 等. 英诺克李斯特菌侵染果蝇S2细胞后的转录组分析[J]. 华南农业大学学报, 2020, 41(5): 17-26. DOI: 10.7671/j.issn.1001-411X.202001023
    shu
    WU Kunzhong, LI Rongsong, CAO Yang, et al. Transcriptome analysis of Drosophila S2 cells infected by Listeria innocua[J]. Journal of South China Agricultural University, 2020, 41(5): 17-26. DOI: 10.7671/j.issn.1001-411X.202001023
    Citation: WU Kunzhong, LI Rongsong, CAO Yang, et al. Transcriptome analysis of Drosophila S2 cells infected by Listeria innocua[J]. Journal of South China Agricultural University, 2020, 41(5): 17-26. DOI: 10.7671/j.issn.1001-411X.202001023
    shu

    英诺克李斯特菌侵染果蝇S2细胞后的转录组分析

    Transcriptome analysis of Drosophila S2 cells infected by Listeria innocua

      摘要
    • 摘要:
      目的  英诺克李斯特菌Listeria innocua为李斯特菌属Listeria的一种非致病菌，与致病菌单增李斯特菌L. monocytogenes具有共同的毒力祖先，本文旨在研究英诺克李斯特菌侵染后宿主基因表达的变化情况，为宿主调控、防御单增李斯特菌的危害提供参考。
      方法  用英诺克李斯特菌侵染果蝇S2细胞，利用转录组测序检测该细菌侵染后果蝇S2细胞的基因表达变化，采用qPCR验证英诺克李斯特菌侵染后果蝇S2细胞中差异表达的基因。
      结果  英诺克李斯特侵染3 h后，宿主果蝇S2细胞中Toll/Imd信号通路发生显著上调，吞噬体和霍乱弧菌Vibrio cholerae感染信号通路显著下调；抗菌肽基因DmDef(DmDefensin)、DmDro(DmDrosomycin)、DmDpt A(DmDptericin A)、DmDpt B(DmDptericin B)、DmMtk(DmMetchnikowin)、DmCec A2(DmCecropin A2)、DmAtt A(DmAttacin A)、DmAtt B(DmAttacin B)、DmAtt D(DmAttacin D)、DmCec B(DmCecropin B)均被显著地诱导表达，其中，上调幅度最大的基因是DmDef，上调了9.805倍。qPCR验证结果显示，DmMtk、DmAtt A、DmDro和DmDef基因表达分别上调了8.180、7.533、7.204和4.569倍。
      结论  英诺克李斯特菌侵染果蝇S2细胞后，变化最显著的基因类群为抗菌肽。本研究全面调查了英诺克李斯特菌侵染后宿主基因表达的变化情况，为揭示非致病菌侵染后宿主细胞的反应、细菌与宿主的互作规律提供了参考。

       

      Abstract:
      Objective  Listeria innocua is a non-pathogenic bacterium from the Listeria genus, which harbors the virulence factors evolved from the same ancestor with the pathogenic bacterium L. monocytogenes. This study aims to investigate the transcriptional variations of host cells after L. innocuainfection, and provide a basis for host regulation and prevention of damage from L. monocytogenes.
      Method  We used L. innocua to infect Drosophila melanogaster S2 cells and analyzed the change of gene expression in Drosophila S2 cells by transcriptome sequencing. The differentially expressed genes in Drosophila S2 cells infected by L. innocua was verified by qPCR.
      Result  The Toll/Imd signaling pathways were significantly upregulated in Drosophila S2 cells after L. innocua infection for three hours, while the phagosome and Vibrio cholerae infection signaling pathways were significantly downregulated. The antimicrobial peptide genes including DmDef (DmDefensin), DmDro (DmDrosomycin), DmDpt A (DmDptericin A), DmDpt B (DmDptericin B), DmMtk (DmMetchnikowin), DmCec A2 (DmCecropin A2), DmAtt A (DmAttacin A), DmAtt B (DmAttacin B), DmAtt D (DmAttacin D), and DmCec B (DmCecropin B) were significantly induced after L. innocua infection. Among them, the most upregulated gene was DmDef with 9.805 fold change. The qPCR verification results showed that the expressions of DmMtk, DmAtt A, DmDro and DmDef genes were upregulated by 8.180, 7.533, 7.204 and 4.569 fold.
      Conclusion  After L. innocua infection, the genes with the most significant change in Drosophila S2 cells are antimicrobial peptide genes. This study offers a comprehensive investigation of gene expression changes in Drosophila S2 cells after L. innocua infection, and provide a reference for revealing the response of host cells evoked by non-pathogenic bacteria as well as interaction studies between bacterial pathogens and hosts.

       

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