Relationship between soil methane emission flux and active organic carbon content in sugarcane field under drip fertigation
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摘要:目的
研究蔗田滴灌施肥对土壤活性有机碳含量和甲烷排放通量的影响，探讨蔗田滴灌施肥土壤甲烷排放通量与土壤活性有机碳含量之间的关系。
方法2018年3—12月在南宁市灌溉试验站开展不同滴灌灌水、施肥的田间试验，试验设4种施肥水平：常规施肥(F100，N 250 kg·hm−2、P2O5 150 kg·hm−2、K2O 200 kg·hm−2)、增量施肥1(F110，在F100基础上增加10%)、增量施肥2(F120，在F100基础上增加20%)和减量施肥(F90，在F100基础上减少10%)，以及2种滴灌灌水水平：W180(180 m3·hm−2)和W300(300 m3·hm−2)。用常规法测定不同生育时期蔗田土壤甲烷排放通量和土壤活性有机碳含量，用Pearson法分析土壤甲烷排放通量与土壤活性有机碳含量的关系。
结果在分蘖期，W300F120处理土壤可溶性有机碳(DOC)含量较W300F100提高了156%，而土壤CH4排放通量较其他处理低。在成熟期，W300F120处理土壤DOC含量较W300F110增加了114%，微生物量碳(MBC)较W300F110增加了49.6%。蔗田土壤CH4排放通量仅与土壤DOC含量呈显著正相关，相关系数为0.38。
结论土壤DOC含量显著影响蔗田土壤甲烷排放通量。W300F120处理可以提高分蘖期和成熟期蔗田土壤可溶性有机碳含量、减少分蘖期蔗田土壤CH4排放。
Abstract:ObjectiveTo study the effects of drip fertigation on soil active organic carbon content and methane (CH4) emission flux in sugarcane field, and investigate the relationship between soil methane emission flux and soil active organic carbon content.
MethodThe field experiment with different irrigation and fertilization treatments under drip irrigation was conducted in Nanning Irrigation Experimental Station from March to December in 2018. Four fertilization levels were designed: Conventional fertilization (F100, N 250 kg·hm−2, P2O5 150 kg·hm−2, K2O 200 kg·hm−2), incremental fertilization 1 (F110, 10% increase based on F100), incremental fertilization 2 (F120, 20% increase on the basis of F100), and reducing fertilization (F90, 10% reduction based on F100). Two drip irrigation levels were set: W180 (180 m3·hm−2) and W300 (300 m3·hm−2). Soil CH4 emission flux and active organic carbon contents at different growth stages of sugarcane were measured using conventional method, and the relationships between soil CH4 emission flux and active organic carbon contents in sugarcane field were analyzed by Pearson method.
ResultAt tillering stage, W300F120 treatment increased soil soluble organic carbon (DOC) content by 156% compared with W300F100 but had lower CH4 emission flux in soil than the other treatments. At maturing stage, W300F120 treatment increased soil DOC content and microbial biomass carbon (MBC) by 114% and 49.6% compared with W300F100, respectively. CH4 emission flux in sugarcane field was only positively correlated with soil DOC content, with the correlation coefficient of 0.38.
ConclusionSoil DOC content significantly affects CH4 emission flux in sugarcane field. W300F120 treatment can increase soil soluble organic carbon content in sugarcane field at tillering and maturing stages, and reduce soil CH4 emission in sugarcane field at tillering stage.
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图 1 试验期间月均温和月降雨量
Figure 1. Monthly average temperature and rainfall during experimental period
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图 2 不同生育期蔗田土壤甲烷排放通量
SS: 苗期，TS：分蘖期，ES：伸长期，RS：成熟期；相同生育期不同柱子上，凡是有一个相同小写字母者表示差异不显著(P>0.05，Duncan’s法)
Figure 2. Soil CH4 emission flux at different growth stages in sugarcane field
SS:Seedling stage；TS: Tillering stage；ES: Elongating stage；RS: Ripening stage. The same lowercase letters at the same growth stage indicated no significant difference (P>0.05, Duncan’s test)
表 1 蔗田各生育期灌水量
Table 1 Irrigation amount at different growth stages in sugarcane field
m3·hm−2 生育期
Growth stage灌溉水平 Irrigation level W180 W300 苗期 Seedling stage 60 90 分蘖期 Tillering stage 30 60 伸长期 Elongating stage 60 120 成熟期 Ripening stage 30 30 合计 Total 180 300 
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表 2 不同滴灌施肥处理土壤易氧化有机碳含量1)
Table 2 Content of soil labile organic carbon in different drip fertigation treatments
g·kg−1 滴灌灌水水平
Drip irrigation level施肥水平
Fertilization level苗期
Seedling stage分蘖期
Tillering stage伸长期
Elongating stage成熟期
Ripening stageW180 F100 1.59±0.16a 1.51±0.16bcd 1.25±0.16d 1.23±0.08d F110 1.51±0.34a 1.94±0.01a 1.23±0.12d 1.45±0.03b F120 1.56±0.07a 1.74±0.07ab 1.60±0.15ab 1.67±0.08a F90 1.52±0.30a 1.59±0.14bcd 1.74±0.11a 1.47±0.06b W300 F100 1.23±0.30a 1.29±0.04d 1.31±0.14cd 1.32±0.06cd F110 1.38±0.37a 1.40±0.07cd 1.54±0.18abc 1.38±0.05bc F120 1.20±0.12a 1.64±0.10bc 1.36±0.06bcd 1.43±0.10bc F90 1.61±0.16a 1.43±0.01cd 1.25±0.18d 1.70±0.06a 1) 表中数据为平均值±标准误，同列数据后的不同小写字母表示差异显著 (P<0.05，Duncan’s 法)
1) The values in the table are mean ± standard error, and different lowercase letters in the same column indicated significant difference (P<0.05, Duncan’s test)
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表 3 不同滴灌施肥处理的土壤微生物量碳含量1)
Table 3 Content of soil microbial biomass carbon in different drip fertigation treatments
g·kg−1 滴灌灌水水平
Drip irrigation level施肥水平
Fertilization level苗期
Seedling stage分蘖期
Tillering stage伸长期
Elongating stage成熟期
Ripening stageW180 F100 1.32±0.14b 0.66±0.31a 0.16±0.03c 1.40±0.21bc F110 1.31±0.19b 0.67±0.25a 0.64±0.09a 1.02±0.11c F120 1.27±0.16b 0.41±0.23a 0.65±0.03a 1.46±0.14abc F90 1.26±0.38b 0.54±0.16a 0.25±0.08bc 1.87±0.04a W300 F100 1.82±0.03a 0.64±0.16a 0.38±0.05b 1.30±0.20bc F110 1.40±0.29ab 0.63±0.08a 0.81±0.09a 1.15±0.11c F120 1.31±0.34b 0.25±0.19a 0.41±0.05b 1.72±0.11ab F90 1.67±0.05ab 0.31±0.29a 0.67±0.09a 1.71±0.10ab 1) 表中数据为平均值±标准误，同列数据后的不同小写字母表示差异显著 (P<0.05，Duncan’s 法)
1) The values in the table are mean ± standard error, and different lowercase letters in the same column indicated significant difference (P<0.05, Duncan’s test)
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表 4 不同滴灌施肥处理的土壤可溶性有机碳含量1)
Table 4 Content of soil dissolved organic carbon in different drip fertigation treatments
g·kg−1 滴灌灌水水平
Drip irrigation level施肥水平
Fertilization level苗期
Seedling stage分蘖期
Tillering stage伸长期
Elongating stage成熟期
Ripening stageW180 F100 0.12±0.06a 0.29±0.11cd 0.14±0.06b 0.12±0.02c F110 0.28±0.10a 0.43±0.03abcd 0.15±0.07b 0.23±0.03bc F120 0.31±0.15a 0.50±0.05abc 0.15±0.05b 0.30±0.14abc F90 0.13±0.05a 0.41±0.12bcd 0.30±0.06a 0.34±0.14ab W300 F100 0.10±0.08a 0.25±0.15d 0.15±0.09b 0.21±0.09bc F110 0.28±0.13a 0.57±0.20ab 0.23±0.12ab 0.23±0.12bc F120 0.26±0.10a 0.64±0.13a 0.18±0.05ab 0.45±0.18a F90 0.24±0.17a 0.21±0.07d 0.16±0.08ab 0.51±0.08a 1) 表中数据为平均值±标准误，同列数据后的不同小写字母表示差异显著 (P<0.05，Duncan’s 法)
1) The values in the table are mean ± standard error, and different lowercase letters in the same column indicated significant difference (P<0.05, Duncan’s test)
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