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    王璋强, 李蕊芯, 陆怡, 等. 展颖野生稻单片段代换系芽期耐Cd材料筛选及QTL鉴定[J]. 华南农业大学学报, 2020, 41(1): 69-77. doi: 10.7671/j.issn.1001-411X.201905023
    引用本文: 王璋强, 李蕊芯, 陆怡, 等. 展颖野生稻单片段代换系芽期耐Cd材料筛选及QTL鉴定[J]. 华南农业大学学报, 2020, 41(1): 69-77. doi: 10.7671/j.issn.1001-411X.201905023
    shu
    WANG Zhangqiang, LI Ruixin, LU Yi, et al. Cd-tolerant germplasm screen and QTL identification of single-segment substitution lines of Oryza glumaepatula at the germination period[J]. Journal of South China Agricultural University, 2020, 41(1): 69-77. doi: 10.7671/j.issn.1001-411X.201905023
    Citation: WANG Zhangqiang, LI Ruixin, LU Yi, et al. Cd-tolerant germplasm screen and QTL identification of single-segment substitution lines of Oryza glumaepatula at the germination period[J]. Journal of South China Agricultural University, 2020, 41(1): 69-77. doi: 10.7671/j.issn.1001-411X.201905023
    shu

    展颖野生稻单片段代换系芽期耐Cd材料筛选及QTL鉴定

    Cd-tolerant germplasm screen and QTL identification of single-segment substitution lines of Oryza glumaepatula at the germination period

      摘要
    • 摘要:
      目的  评价CdCl2溶液对展颖野生稻Oryza glumaepatula单片段代换系(SSSLs)种子发芽及根芽生长的影响，筛选耐Cd SSSLs材料，初步鉴定与芽期耐Cd性状相关的QTLs。
      方法  对6个栽培稻品种在0、30、50、100、500 μmol·L−1 CdCl2溶液条件下进行发芽试验，确定水稻种子芽期耐Cd性研究的处理浓度，筛选耐Cd SSSLs的性状指标。对SSSLs在CdCl2溶液处理下发芽第7天的根长与芽长分析，筛选P=0.01水平耐Cd性较强的SSSLs，鉴定相应性状QTLs。
      结果  在0、30、50和100 μmol·L−1 CdCl2浓度处理下，栽培稻品种的种子发芽率为91%~95%，差异不显著，但种子根和幼芽伸长生长受到抑制，品种间差异显著。在50和100 μmol·L−1 CdCl2溶液条件下，以发芽第7天的相对根长和相对芽长为指标，在P=0.01水平从8个SSSLs鉴定出5个相对根长QTLs，即qRRL1-1、qRRL2-1、qRRL3-1、qRRL3-2及qRRL6-1，其加性效应为0.19~0.60，表型贡献率为31.18%~100.59%；鉴定出5份SSSLs携带3个相对芽长QTLs，即qRSL1-1、qRSL1-2及qRSL2-1，其加性效应为0.08~0.18，表型贡献率为7.43%~18.95%。
      结论  展颖野生稻SSSLs携带芽期耐Cd QTLs，可以作为水稻耐Cd基因发掘的重要材料。

       

      Abstract:
      Objective  To evaluate effects of Cd stress on seed germination and growth of seminal root and shoot in chromosome single-segment substitution lines (SSSLs) of Oryza glumaepatula after seed germination, screen for Cd-tolerant SSSLs, and identify QTLs related to Cd-tolerant characters in bud.
      Method  The seed germination test was carried out using six varieties at CdCl2 concentrations of 0, 30, 50, 100, 500 μmol·L−1, and the proper treatment of CdCl2 concentration and the related character indexes to evaluate CdCl2 tolerance of SSSLs were determined. The lengths of seminal roots and shoots of experimental SSSLs were analyzed to screen for SSSLs with strong tolerance to CdCl2 at P=0.01 level and identify QTLs related to Cd-tolerant characters.
      Result  At the concentrations of 0, 30, 50, and 100 μmol·L−1 CdCl2, the seed germination rates of six varieties were distributed between 91%～95%, which were insignificant among different CdCl2 concentrations. However, the growth of seminal roots and shoots were significantly inhibited by CdCl2, and the significant differences were observed for the tolerance to CdCl2 among different varieties. Using relative root length and relative shoot length on the 7th day after germination as tolerance indexes, at the concentrations of 50 and 100 μmol·L−1, five relative root length QTLs including qRRL1-1, qRRL2-1, qRRL3-1, qRRL3-2 and qRRL6-1 were identified in eight SSSLs at P=0.01 level, the phenotypic contribution rates of additive effects ranged from 31.18% to 100.59%. Three relative shoot length QTLs including qRSL1-1, qRSL1-2, and qRSL2-1 were identified in five SSSLs, the phenotypic contribution rates of additive effects were 7.43%～18.95%.
      Conclusion  SSSLs of O. glumaepatula carry Cd-tolerant QTLs at the germination period, which will be useful for exploring beneficial Cd-tolerant genes in rice.

       

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