Metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities
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摘要:目的
研究小菜蛾共生真菌棒曲霉Aspergillus clavatus XCE02代谢产物及其抗植物病原真菌的活性。
方法柱层析技术分离纯化XCE02代谢产物，并运用波谱技术鉴定结构；滤纸片扩散法测试代谢产物对香蕉炭疽菌和小麦赤霉菌的抑菌活性。
结果分离鉴定出gliomasolide A、Sch725674、gliomasolide C、clavatustide A、clavatustide B、20−羟基麦角甾−4，6，8(14)，22−四烯−3−酮、黄嘌呤、麦角甾醇、过氧化麦角甾醇和丁二酸10个化合物。在250 μg/mL时，gliomasolide A、Sch725674和gliomasolide C对香蕉炭疽菌和小麦赤霉菌显示了高度抗菌活性，clavatustide A和clavatustide B对香蕉炭疽菌和小麦赤霉菌显示了中度抗菌活性，20−羟基麦角甾−4，6，8(14)，22−四烯−3−酮对小麦赤霉菌显示了中度抗菌活性。
结论从曲霉属分离到了gliomasolide A、gliomasolide C和20−羟基麦角甾−4，6，8(14)，22−四烯−3−酮，gliomasolide A、Sch725674和gliomasolide C可作为相应抗菌农药先导化合物开展深入研究。
Abstract:ObjectiveTo study the metabolites of a symbiotic fungus Aspergillus clavatus XCE02 from Plutella xylostella and their antifungal activities against plant pathogens.
MethodThe metabolites were isolated and purified by column chromatography. Their structures were elucidated by spectral analysis. The antifungal activities of the metabolites against Colletotrichum musae and Fusarium graminearum were investigated using paper disc-agar diffusion method.
ResultTen compounds were isolated and identified as gliomasolide A, Sch725674, gliomasolide C, clavatustide A, clavatustide B, 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one, xanthine, ergosterol, ergosterol peroxide and butanedioic acid. At the concentration of 250 μg/mL, gliomasolide A, Sch725674 and gliomasolide C exhibited high inhibitory activities against C. musae and F. graminearum, clavatustide A and clavatustide B showed moderate inhibitory activities against C.musae and F.graminearum and 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one showed moderate inhibitory activity against F.graminearum.
ConclusionGliomasolide A, gliomasolide C and 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one have been obtained from the genus Aspergillus in this study. Gliomasolide A, Sch725674 and gliomasolide C can be used as leading compounds for developing antifungal pesticide.
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表 1 化合物的抗植物病原菌活性1)
Table 1 Antifungal activities of compounds against plant pathogens
测试化合物 Tested compound d(抑菌圈 Inhibition zone)/mm 香蕉炭疽菌 Calletotrichum musae 小麦赤霉菌 Fusarium graminearum gliomasolide A 18.19±0.43eE 15.45±0.41eE Sch725674 17.18±0.36dD 17.02±0.35gG gliomasolide C 18.18±0.46eE 16.22±0.54fF clavatustide A 13.75±0.42cC 13.46±0.36cC clavatustide B 13.66±0.24cC 14.47±0.27dD 20−羟基麦角甾−4, 6, 8(14), 22−四烯−3−酮 10.54±0.43bB 12.37±0.53bB 20-hydroxyergosta-4, 6, 8(14), 22-tetraen-3-one 多菌灵 Positive control 26.53±0.20fF 18.57±0.18hH 空白对照 Negative control 0aA 0aA 1)表中数据为平均值±标准误，同列数据后不同小写字母表示组间0.05水平差异显著，不同大写字母表示组间0.01水平差异显著(Duncan’ s法)
1)The data in the table are average values ± standard errors, and different lowercase letters in the same column indicate significant differences among groups at 0.05 level, different capital letters in the same column indicate significant differences among groups at 0.01 level (Duncan’ s method)
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