• 《中国科学引文数据库(CSCD)》来源期刊
  • 中国科技期刊引证报告(核心版)期刊
  • 《中文核心期刊要目总览》核心期刊
  • RCCSE中国核心学术期刊

高蛋白饲粮致雏鹅痛风的临床病理学研究

李曼曼, 丁雪东, 荣雪路, 方天仪, 骞守法, 贺濛初, 李玉, 李锦春, 吴金节

李曼曼, 丁雪东, 荣雪路, 等. 高蛋白饲粮致雏鹅痛风的临床病理学研究[J]. 华南农业大学学报, 2019, 40(1): 46-52. DOI: 10.7671/j.issn.1001-411X.201805003
引用本文: 李曼曼, 丁雪东, 荣雪路, 等. 高蛋白饲粮致雏鹅痛风的临床病理学研究[J]. 华南农业大学学报, 2019, 40(1): 46-52. DOI: 10.7671/j.issn.1001-411X.201805003
LI Manman, DING Xuedong, RONG Xuelu, et al. Clinical pathological study of gosling gout induced by high protein diet[J]. Journal of South China Agricultural University, 2019, 40(1): 46-52. DOI: 10.7671/j.issn.1001-411X.201805003
Citation: LI Manman, DING Xuedong, RONG Xuelu, et al. Clinical pathological study of gosling gout induced by high protein diet[J]. Journal of South China Agricultural University, 2019, 40(1): 46-52. DOI: 10.7671/j.issn.1001-411X.201805003

高蛋白饲粮致雏鹅痛风的临床病理学研究

基金项目: 国家重点研发计划(2016YFD0501205);安徽农业大学研究生创新基金项目(2018-yjs-21)
详细信息
    作者简介:

    李曼曼(1991—),女,硕士研究生,E-mail: 18317715009@163.com

    通讯作者:

    吴金节(1962—),男,教授,硕士,E-mail: wjj@ahau.edu.cn

  • 中图分类号: S835

Clinical pathological study of gosling gout induced by high protein diet

  • 摘要:
    目的 

    研究高蛋白饲粮对1~14日龄雏鹅生长性能、血液生化指标及肝脏、肾脏组织的影响,并对其痛风致病机理进行初步探讨。

    方法 

    选用体质量相近的1日龄雏雁鹅72只,随机分成3组:A、B和C组分别饲喂粗蛋白质量分数为16%、20%和24%的饲粮,每组3个重复,每个重复8只。

    结果 

    A组鹅的生长性能高于B、C组;血清总蛋白、白蛋白和球蛋白的水平随着饲料蛋白水平增加而升高,但差异不显著;C组血清尿酸、丙氨酸氨基转移酶、尿素氮、乳酸脱氢酶的水平显著高于A、B组,而C组总胆红素水平显著低于A、B组。C组肝脏组织中的白细胞介素–1β、白细胞介素–8、黄嘌呤氧化酶活性和肾脏中肿瘤坏死因子–α活性显著高于A、B组。A组肝脏、肾脏组织正常;B组肝脏组织有轻微炎性细胞,肾脏组织正常;C组肝脏细胞出现坏死、水肿和炎性浸润,肾小管空泡变性,肾小球萎缩。C组鹅出现痛风症状。

    结论 

    在饲养条件相同的情况下,饲喂粗蛋白质量分数为24%的饲粮影响雏鹅的生长性能和血清尿酸等部分血液生化指标,引起肝脏和肾脏组织一定程度的损伤以及输尿管有尿酸盐沉积等痛风变化。

    Abstract:
    Objective 

    To study the effects of high protein diet on growth performance, blood biochemical parameters, liver and kidney tissues of goslings at the age of 1 to 14 days, and preliminarily investigate gout pathogenesis of high protein diet.

    Method 

    Seventy-two one-day-old geese were randomly allocated into three groups with three replicates per group and eight geese per replicate. Group A, B and C were fed with diets containing 16%, 20% and 24% crude protein, respectively.

    Result 

    The growth performance of geese in group A were higher than those in group B and C. Total protein, albumin and globulin levels of serum increased with the increase of dietary protein level, but the differences were not significant. Serum uric acid, alanine aminotransferase, urea nitrogen, and lactate dehydrogenase levels in group C were significantly higher than those in group A and B, while total bilirubin level in group C was significantly lower than those in group A and B. The interleukin-1β, interleukin-8 and xanthine oxidase activities of liver tissues and tumor necrosis factor-α activity of kidney tissues in group C were significantly higher than those in group A and B. In group A, the liver and kidney tissues were normal. In group B, there were mild inflammatory cells in liver tissues, and the kidney tissues were normal. In group C, necrosis, edema and inflammatory infiltration of liver cells and tubular vacuolar degeneration, glomerular atrophy of kidney were observed. The geese exhibited gout symptoms in group C.

    Conclusion 

    Under the same feeding conditions, feeding with 24% crude protein can affect growth performance, serum uric acid and other blood biochemical indexes of goslings, cause some damage to liver and kidney tissue, and produce gout symptoms, such as urate deposition in the ureter.

  • 图  1   鹅肝脏和肾脏HE染色后的显微照片

    a、e分别为A组鹅的肝脏和肾脏组织结构;b、f分别为B组鹅的肝脏和肾脏组织结构;c、d均为C组鹅的肝脏组织结构;g、h均为C组鹅的肾脏组织结构

    Figure  1.   Microscopic pictures of goose liver and kidney after HE staining

    a and e were liver and kidney tissue structures in geese of group A, respectively; b and f were liver and kidney tissue structures in geese of group B, respectively; c and d were liver tissue structures in geese of group C; g and h were kidney tissue structures in geese of group C

    图  2   鹅肝脏、肾脏透射电镜图片

    a、b和c分别为A、B和C组肝脏细胞结构;d、e和f分别为A、B和C组肾小管上皮细胞结构

    Figure  2.   Transmission electron microscope pictures of goose liver and kidney

    a, b and c were liver cell structures in group A, B and C, respectively; d, e and f were renal tubular epithelial cell structures in group A, B and C, respectively

    表  1   试验饲粮营养组成与含量1)

    Table  1   Nutrient compositions and levels of experimental diets

    项目 Item 组成 Component 组别 Group
    A B C
    原料及质量分数/%
    Ingredient content
    玉米 Corn 46.00 42.00 36.45
    小麦次粉 Wheat midding 15.00 13.90 13.79
    米糠 Rice bran 20.00 18.53 17.06
    蛋白粉 Albumen powder 0 7.35 14.70
    玉米胚芽粕 Corn germ meal-so1 4.00 4.00 4.00
    酒糟蛋白饲料 Distillers dried grains with solubles 9.00 8.22 8.00
    石粉 Stone powder 0.72 0.72 0.72
    玉米蛋白粉 Corn gluten meal 3.00 3.00 3.00
    预混料1) Premix 2.28 2.28 2.28
    合计 Total 100.00 100.00 100.00
    营养成分及质量分数/%
    Nutrient content
    粗蛋白质2) Crude protein 16.00 20.00 24.00
    钙 Ca 1.10 1.10 1.10
    有效磷 Available phosphorous 0.65 0.65 0.68
    粗纤维 Crude fiber 6.80 6.60 6.40
    粗灰分 Ash 9.00 9.00 9.10
    氯化钠 NaCl 0.50 0.50 0.50
    水分 Water 13.00 13.00 13.00
    蛋氨酸 DL-Methionine 0.50 0.51 0.52
    代谢能/(MJ·kg–1)
    Metabolic energy
    2.70 2.69 2.70
     1) 预混料为每千克饲料提供: 维生素A 1.4×105 IU, 维生素D3 6×104 IU, 维生素E 100 IU, 维生素K3 28 mg, 维生素B1 12 mg, 维生素B2 62 mg, 维生素B6 18 mg, 维生素B12 0.12 mg, 维生素B5 100 mg,维生素B3 3 180 mg,维生素B9 11.6 mg,维生素B7 1.6 mg,胆碱 5 000 mg,Mn 900 mg,Zn 1 000 mg,Fe 1.3 g,Cu 250 mg,Se 4.5 mg,I 7 mg,赖氨酸 25 000 mg,w(Ca) 9%,w(P) 3%,w(H2O) 12%
     1) The premix provided the following per kilogram of diet: Vitamin A 1.4×105 IU, vitamin D3 6×104 IU, vitamin E 100 IU, vitamin K3 28 mg, vitamin B1 12 mg, vitamin B2 62 mg, vitamin B6 18 mg, vitamin B12 0.12 mg, vitamin B5 100 mg,vitamin B3 3 180 mg,vitamin B9 11.6 mg,vitamin B7 1.6 mg,choline 5 000 mg,Mn 900 mg,Zn 1 000 mg,Fe 1.3 g,Cu 250 mg,Se 4.5 mg,I 7 mg, Lys 25 000 mg,w(Ca) 9%,w(P) 3%,w(H2O) 12%
    下载: 导出CSV

    表  2   不同试验组鹅体重与料重比变化

    Table  2   Changes in the body weight and feed weight ratio of goose in different experimental group

    组别
    Group
    体质量/g1)  Body weight 料重比/%  Feed weight ratio
    第1天
    1st day
    第7天
    7th day
    第14天
    14th day
    第1~7天
    1st to 7th day
    第8~14天
    8th to 14th day
    A 124.00±3.10a 258.57±15.52a 398.46±71.99a 2.29 1.79
    B 124.43±3.10a 226.77±28.99a 327.17±53.26a 2.83 2.37
    C 124.63±2.62a 181.00±39.88b 270.25±31.64b 3.20 3.32
     1) 同列数据后不同小写字母表示差异显著 (P<0.05, Duncan’s法)
     1) Different lowercase letters in the same column indicated significant difference (P<0.05, Duncan’s test)
    下载: 导出CSV

    表  3   14日龄雏鹅血清生化指标1)

    Table  3   Serum biochemical indicators of 14-day-old goslings

    项目 Item 组别 Group
    A B C
    丙氨酸氨基转移酶活性/(U·L–1) Alanine aminotransferase activity 18.80±3.53b 19.69±6.83b 50.72±38.36a
    天冬氨酸氨基转移酶活性/(U·L–1) Aspartate aminotransferase activity 15.20±6.38a 23.24±15.71a 25.68±7.95a
    碱性磷酸酶活性/(U·L–1) Alkaline phosphatase activity 771.14±276.49a 698.95±331.51a 714.40±326.05a
    γ−谷氨酰转移酶活性/(U·L–1)  γ-glutaminyl transferase activity 2.17±2.75a 2.87±3.75a 4.05±1.14a
    乳酸脱氢酶活性/(U·L–1) Lactate dehydrogenase activity 201.04±56.06b 355.66±109.74b 821.59±363.56a
    ρ(总蛋白) /(g·L–1) Total protein content 36.64±6.92a 35.31±4.93a 32.51±6.62a
    ρ(白蛋白) /(g·L–1) Albumin content 14.01±1.38a 14.09±1.77a 13.78±1.59a
    ρ(球蛋白) /(g·L–1) Globulin content 22.61±6.94a 21.21±4.76a 18.73±6.63a
    c(尿酸) /(μmol·L–1) Uric acid concentration 142.33±23.49b 133.88±57.05b 230.27±82.81a
    c(总胆红素) /(μmol·L–1) Total bilirubin concentration 12.07±2.21a 8.86±2.33b 4.81±5.10b
    c(尿素氮) /(mmol·L–1) Urea nitrogen concentration 0.40±0.32b 0.43±0.14b 1.12±0.58a
     1) 同行数据后不同小写字母表示差异显著 (P<0.05, Duncan’s法)
     1) Different lowercase letters in the same row indicated significant difference (P<0.05, Duncan’s test)
    下载: 导出CSV

    表  4   不同试验组鹅肝脏中炎症因子水平1)

    Table  4   The inflammatory cytokine level of goose liver in different experimental group

    项目 Item 组别 Group
    A B C
    ρ(IL-1β)/(pg·mL–1) 465.37±45.49b 495.09±39.27b 500.76±32.48a
    ρ(IL-6)/(pg·mL–1) 290.53±25.85a 295.99±24.67a 343.03±33.73a
    ρ(IL-8)/(pg·mL–1) 1 168.45±49.59b 1 238.39±145.69b 1 421.43±257.08a
    ρ(TNF-α)/(pg·mL–1) 1 775.58±113.00a 1 834.83±178.47a 1 842.25±131.31a
    ρ(TNF-β)/(pg·mL–1) 1 775.58±113.00a 1 834.83±178.47a 1 842.25±131.31a
    黄嘌呤氧化酶活性/(U·L–1) Xanthine oxidase activity 40.93±7.33b 49.17±2.80b 51.53±2.96a
     1) 同行数据后不同小写字母表示差异显著 (P<0.05, Duncan’s法)
     1) Different lowercase letters in the same row indicated significant difference (P<0.05, Duncan’s test)
    下载: 导出CSV

    表  5   不同试验组鹅肾脏中炎症因子水平1)

    Table  5   The inflammatory cytokines level of goose kidney in different experimental group

    项目 Item 组别 Group
    A B C
    ρ(IL-1β)/(pg·mL–1) 571.05±24.53a 570.01±60.90a 556.57±31.57a
    ρ(IL-6)/(pg·mL–1) 290.0±17.10a 316.94±28.01a 328.24±20.40a
    ρ(IL-8)/(pg·mL–1) 1 086.61±19.21a 1 114.88±56.94a 1 114.64±42.77a
    ρ(TNF-α)/(pg·mL–1) 1 484.11±68.87b 1 517.44±154.03b 1 703.23±98.99a
    ρ(TNF-β)/(pg·mL–1) 1 516.36±126.38a 1 537.44±107.06a 1 624.11±107.43a
    黄嘌呤氧化酶活性/(U·L–1) Xanthine oxidase activity 52.10±1.97a 52.57±2.39a 53.09±2.65a
     1) 同行数据后不同小写字母表示差异显著 (P<0.05, Duncan’s法)
     1) Different lowercase letters in the same row indicated significant difference (P<0.05, Duncan’s test)
    下载: 导出CSV
  • [1] 许英民, 杨成勇. 肉仔鹅饲喂肉鸡料引发痛风病的报告[J]. 中国禽业导刊, 2006, 23(11): 25.
    [2] 李涌. 家禽发生痛风病因和防治[J]. 农业开发与装备, 2017(5): 187. doi: 10.3969/j.issn.1673-9205.2017.05.160
    [3]

    SINGH N, GHOSH R C. Pathology of gout induced by high protein diets in growing broilers[J]. Asian J Animal Sci, 2014, 9(2): 149-153. doi: 10.15740/HAS/TAJAS

    [4] 费强. 一起笼养蛋鸡痛风病的诊治[J]. 家禽科学, 2015(9): 31-32. doi: 10.3969/j.issn.1673-1085.2015.09.010
    [5] 王铁良, 李永久, 马有为. 雏鸡痛风的原因及防治[J]. 中国畜牧兽医, 2010, 37(4): 229-231.
    [6] 陈鲜鑫, 刘震坤. 浅析禽痛风的发病原因及防治方法[J]. 兽医导刊, 2017(6): 116.
    [7] 常斌, 王润莲, 庞华琦, 等. 肉鹅营养需要研究进展[J]. 饲料工业, 2008, 29(13): 26-28. doi: 10.3969/j.issn.1001-991X.2008.13.008
    [8] 贺普霄. 家畜营养代谢病[M]. 北京: 中国农业出版社, 1994: 40-41.
    [9] 刘五岳. 鹅的饲养标准[J]. 畜禽业, 2003(10): 11. doi: 10.3969/j.issn.1008-0414.2003.10.004
    [10]

    VAN BEEK J H, DE MOOR M H, DE GEUS E J, et al. The genetic architecture of liver enzyme levels: GGT, ALT and AST[J]. Behav Genet, 2013, 43(4): 329-339. doi: 10.1007/s10519-013-9593-y

    [11]

    HÜGLE T, KRENN V. Histopathophysiology of gout[J]. Ther Umsch, 2016, 73(3): 137-140. doi: 10.1024/0040-5930/a000769

    [12]

    NASOORI A, PEDRAM B, KAMYABIMOGHADDAM Z, et al. Clinicopathologic characterization of visceral gout of various internal organs - a study of 2 cases from a venom and toxin research center[J]. Diagn Pathol, 2015, 10(1): 23. doi: 10.1186/s13000-015-0251-y

    [13] 周伟, 谭云, 王小波. 鸡痛风的组织病理学观察[J]. 中国兽医杂志, 2017, 53(4): 42-44.
    [14]

    MARTINON F, PÉTRILLI V, MAYOR A, et al. Gout-associated uric acid crystals activate the NALP3 inflammasome[J]. Nature, 2006, 440(7081): 237-241. doi: 10.1038/nature04516

    [15]

    YEN D, CHEUNG J, SCHEERENS H, et al. IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6[J]. J Clin Invest, 2006, 116(5): 1310-1316. doi: 10.1172/JCI21404

    [16]

    UELAND T, GULLESTAD L, NYMO S H, et al. Inflammatory cytokines as biomarkers in heart failure[J]. Clin Chim Acta, 2015, 443: 71-77. doi: 10.1016/j.cca.2014.09.001

    [17] 郭小权, 曹华斌, 黄克和, 等. 高钙日粮对青年蛋鸡肾脏中XOD基因mRNA表达的影响[J]. 中国兽医学报, 2011, 31(11): 1663-1666.
    [18] 黄继勇, 张艳. 痛风患者外周血中炎症因子IL-18, IL-1β, TNF-α的变化[J]. 中华全科医学, 2017, 15(9): 1487-1489.
  • 期刊类型引用(1)

    1. 张运春,欧春予,张桥英. 氮添加对不同密度入侵植物喜旱莲子草生长的影响. 生态环境学报. 2020(09): 1745-1751 . 百度学术

    其他类型引用(5)

图(2)  /  表(5)
计量
  • 文章访问数:  1331
  • HTML全文浏览量:  4
  • PDF下载量:  1177
  • 被引次数: 6
出版历程
  • 收稿日期:  2018-05-03
  • 网络出版日期:  2023-05-17
  • 刊出日期:  2019-01-09

目录

    /

    返回文章
    返回