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    汪珩, 张秀芬, 钟少颖, 等. 不同粗蛋白质和能量水平对鸭肠液中消化酶活性的影响[J]. 华南农业大学学报, 2018, 39(6): 5-9. doi: 10.7671/j.issn.1001-411X.2018.06.002
    引用本文: 汪珩, 张秀芬, 钟少颖, 等. 不同粗蛋白质和能量水平对鸭肠液中消化酶活性的影响[J]. 华南农业大学学报, 2018, 39(6): 5-9. doi: 10.7671/j.issn.1001-411X.2018.06.002
    shu
    WANG Heng, ZHANG Xiufen, ZHONG Shaoying, ZHU Yongwen, WANG Wence, YANG Lin. Effects of crude protein and energy levels on digestive enzyme activities in duck jejunal fluid[J]. Journal of South China Agricultural University, 2018, 39(6): 5-9. DOI: 10.7671/j.issn.1001-411X.2018.06.002
    Citation: WANG Heng, ZHANG Xiufen, ZHONG Shaoying, ZHU Yongwen, WANG Wence, YANG Lin. Effects of crude protein and energy levels on digestive enzyme activities in duck jejunal fluid[J]. Journal of South China Agricultural University, 2018, 39(6): 5-9. DOI: 10.7671/j.issn.1001-411X.2018.06.002
    shu

    不同粗蛋白质和能量水平对鸭肠液中消化酶活性的影响

    Effects of crude protein and energy levels on digestive enzyme activities in duck jejunal fluid

      摘要
    • 摘要:
      目的  研究日粮蛋白质和能量水平对番鸭空肠液中几种主要消化酶活性的影响。
      方法  采用3×3双因素完全随机设计，将27只24周龄体质量相近的公番鸭随机分为9组，每组3只鸭，分别饲喂9种不同能量蛋白质配比的日粮，通过实施瘘管手术收集空肠液，并测定肠液中淀粉酶、胰蛋白酶、糜蛋白酶和脂肪酶的活性。
      结果  随着能量水平的升高，高、中能量组的胰蛋白酶活性显著高于低能量组(P<0.05)，糜蛋白酶、淀粉酶和脂肪酶活性差异不显著；随着粗蛋白水平的升高，高、中蛋白质组的胰蛋白酶活性显著高于低蛋白组(P<0.05)，高蛋白质组的糜蛋白酶活性显著高于低蛋白质组(P<0.05)，淀粉酶和脂肪酶活性差异不显著。蛋白质和能量水平对番鸭空肠液中几种主要的消化酶活性无明显交互效应。
      结论  本试验条件下，能量水平对胰蛋白酶活性有显著影响，蛋白质水平对胰蛋白酶和糜蛋白酶活性有显著影响，能量和蛋白质水平对淀粉酶和脂肪酶活性均无显著影响。

       

      Abstract:
      Objective  To investigate the effects of dietary protein and energy levels on digestive enzyme activities in the jejunal fluid of Muscovy duck.
      Method  A 3×3 factorial and completely randomized design was conducted. Totally 27 male Muscovy ducks were randomly divided into 9 groups with 3 ducks per group, and fed diets with 9 different compositions of energy and protein. Jejunal digesta was collected by fistula surgery and the activities of amylase, trypsin, chymotrypsin and lipase in jejunal fluid were determined.
      Result  With the increase of energy level, trypsin activities in high and medium energy groups were significantly higher compared with low energy group(P<0.05), while chymotrypsin, amylase and lipase activities did not differ significantly. With the increase of crude protein level, trypsin activities in high and medium protein groups were significantly higher compared with low protein group(P<0.05), chymotrypsin activity in high protein group was significantly higher compared with low protein group(P<0.05), while amylase and lipase activities did not differ significantly. Protein and energy levels had no obvious interaction effect on digestive enzyme activities in the jejunal fluid of Muscovy duck.
      Conclusion  Under this experimental condition, energy level has a significant effect on trypsin activity, and protein level has a significant effect on trypsin and chymotrypsin activities. Neither energy nor protein level has significant effect on amylase and lipase activities.

       

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