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    涂蕊, 但佳明, 严光文, 等. 四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究[J]. 华南农业大学学报, 2018, 39(5): 59-64. doi: 10.7671/j.issn.1001-411X.2018.05.009
    引用本文: 涂蕊, 但佳明, 严光文, 等. 四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究[J]. 华南农业大学学报, 2018, 39(5): 59-64. doi: 10.7671/j.issn.1001-411X.2018.05.009
    shu
    TU Rui, DAN Jiaming, YAN Guangwen, ZHENG Jing, TIAN Yinan, XIAO Qicheng, WEI Bin, ZHOU Ziyao, LIU Haifeng, YANG Tingyu, PENG Guangneng, ZHONG Zhijun. Prevalence and genotyping of Giardia duodenalis in adult goats in Sichuan partial areas[J]. Journal of South China Agricultural University, 2018, 39(5): 59-64. DOI: 10.7671/j.issn.1001-411X.2018.05.009
    Citation: TU Rui, DAN Jiaming, YAN Guangwen, ZHENG Jing, TIAN Yinan, XIAO Qicheng, WEI Bin, ZHOU Ziyao, LIU Haifeng, YANG Tingyu, PENG Guangneng, ZHONG Zhijun. Prevalence and genotyping of Giardia duodenalis in adult goats in Sichuan partial areas[J]. Journal of South China Agricultural University, 2018, 39(5): 59-64. DOI: 10.7671/j.issn.1001-411X.2018.05.009
    shu

    四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究

    Prevalence and genotyping of Giardia duodenalis in adult goats in Sichuan partial areas

      摘要
    • 摘要:
      目的  了解四川省部分地区成年山羊贾第虫Giardia duodenalis感染情况以及感染虫种基因型。
      方法  在四川省12个养殖场采集了342份成年山羊的新鲜粪便，采用多位点基因分型技术，通过巢式PCR扩增bg、tpi和gdh基因位点，对阳性样品的PCR产物进行了测序分析。
      结果  342份成年山羊粪样中贾第虫的总感染率为14.91%(51/342)。所有阳性样品感染的虫种基因型均为集聚体E。51份阳性样品在bg、tpi和gdh这3个基因位点分别成功鉴定出4(E5、E8、E17、E18)、2(E2、E4)和2(E3、E4)种基因亚型，并且在bg位点发现了2种新的基因亚型(E17、E18)。
      结论  四川省部分地区成年山羊存在较为严重的贾第虫感染，虫种类型主要为集聚体E，且具有一定的遗传多样性。

       

      Abstract:
      Objective  To investigate the infection states of Giardia duodenalis and the infected G. duodenalis genotype in adult goats in Sichuan partial areas.
      Method  The fecal samples of 342 adult goats were collected from 12 different farms of Sichuan, and subjected to nested PCR to amplify bg, tpi and gdh gene loci through multilocus genotyping technique. PCR products of positive samples were sequenced and further analyzed.
      Result  The infection rate of G. duodenalis in 342 adult goat fecal samples was 14.91% (51/342) and the infected G. duodenalis genotype in all positive samples was assemblage E. Fifty-one positive samples were respectively identified four (assemblage E5, E8, E17 and E18), two (assemblage E2 and E4), and two (assemblage E3 and E4) subtypes in bg, tpiand gdhgene loci. Two novel genotypes (assemblage E17 and E18) were detected at bg locus.
      Conclusion  There are serious G. duodenalisinfection in adult goats in Sichuan partial areas. The infected G. duodenalisgenotype is mainly assemblage E which has the genetic diversity at a certain level.

       

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