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    朱凌羽, 张子琪, 兰海楠, 等. 虾青素对脂多糖通过TLR4/MyD88/NF-κB信号通路诱导的IPEC-J2细胞炎症的影响[J]. 华南农业大学学报, 2018, 39(5): 53-58. doi: 10.7671/j.issn.1001-411X.2018.05.008
    引用本文: 朱凌羽, 张子琪, 兰海楠, 等. 虾青素对脂多糖通过TLR4/MyD88/NF-κB信号通路诱导的IPEC-J2细胞炎症的影响[J]. 华南农业大学学报, 2018, 39(5): 53-58. doi: 10.7671/j.issn.1001-411X.2018.05.008
    shu
    ZHU Lingyu, ZHANG Ziqi, LAN Hainan, WU Min, LIU Huilin, ZHENG Xin. Effects of astaxanthin on IPEC-J2 cell inflammation induced by lipopolysaccharide via TLR4/MyD88/NF-κB signaling pathway[J]. Journal of South China Agricultural University, 2018, 39(5): 53-58. DOI: 10.7671/j.issn.1001-411X.2018.05.008
    Citation: ZHU Lingyu, ZHANG Ziqi, LAN Hainan, WU Min, LIU Huilin, ZHENG Xin. Effects of astaxanthin on IPEC-J2 cell inflammation induced by lipopolysaccharide via TLR4/MyD88/NF-κB signaling pathway[J]. Journal of South China Agricultural University, 2018, 39(5): 53-58. DOI: 10.7671/j.issn.1001-411X.2018.05.008
    shu

    虾青素对脂多糖通过TLR4/MyD88/NF-κB信号通路诱导的IPEC-J2细胞炎症的影响

    Effects of astaxanthin on IPEC-J2 cell inflammation induced by lipopolysaccharide via TLR4/MyD88/NF-κB signaling pathway

      摘要
    • 摘要:
      目的  研究虾青素(AST)对脂多糖(LPS)通过TLR4/MyD88/NF-κB信号通路诱导的IPEC-J2细胞炎症的影响。
      方法  采用MTT法确定虾青素和LPS对IPEC-J2细胞和转染IPEC-J2细胞的最佳处理浓度和处理时间，在处理后采用实时荧光定量PCR检测IPEC-J2细胞和转染IPEC-J2细胞炎症因子NF-κB、TNF-α、IL-6和IL-1β的mRNA相对表达量，采用ELISA检测上述炎症因子的分泌量。
      结果  当处理3 h，虾青素浓度达到150 μmol·L–1时，IPEC-J2细胞和转染IPEC-J2细胞活力达到峰值；当处理6 h，LPS质量浓度达到100 ng·mL–1时，IPEC-J2细胞和转染IPEC-J2细胞活力达到峰值。与对照组相比，LPS组IPEC-J2细胞NF-κB、TNF-α、IL-6、IL-1β的mRNA相对表达量和分泌量显著升高(P<0.05)；与LPS组相比，AST+LPS组NF-κB、TNF-α、IL-6、IL-1β在IPEC-J2细胞中的mRNA相对表达量和分泌量显著降低(P<0.05)，而在转染IPEC-J2细胞中的炎症因子mRNA相对表达量和分泌量均无显著变化(P>0.05)。
      结论  虾青素可以抑制细胞炎症反应，且对细胞的保护作用与TLR4/MyD88/NF-κB信号通路相关。

       

      Abstract:
      Objective  To investigate the effect of astaxanthin (AST) on IPEC-J2 cell inflammation induced by lipopolysaccharide (LPS) via TLR4/MyD88/NF-κB signaling pathway.
      Method  MTT assay was performed to determine the optimal time and concentrations of astaxanthin and LPS for treating IPEC-J2 cells and transfected IPEC-J2 cells. Fluorescent quantitative RT-PCR was performed to determine the relative mRNA expressions of inflammatory factors including NF-κB, TNF-α, IL-6 and IL-1β in IPEC-J2 cells and transfected IPEC-J2 cells stimulated by LPS. ELISA assays were carried out to determine the secretion amounts of these inflammatory factors.
      Result  The vitality of IPEC-J2 cells and transfected IPEC-J2 cells reached the peak when treated with 150 μmol·L–1 astaxanthin for 3 h or 100 ng·mL–1 lipopolysaccharide for 6 h. Compared with control group, the relative mRNA expressions and secretions of NF-κB, TNF-α, IL-6 and IL-1β in IPEC-J2 cells significantly increased in LPS treatment group (P<0.05). Compared with LPS treatment group, the relative mRNA expressions and secretions of NF-κB, TNF-α, IL-6 and IL-1β in LPS+AST group were significantly lower in IPEC-J2 cells (P<0.05), but did not differ significantly in transfected IPEC-J2 cells (P>0.05).
      Conclusion  Astaxanthin can inhibit cell inflammation, and its protective effect on cells is related to TLR4/MyD88/NF-κB signaling pathway.

       

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