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    李琪, 谢增胜, 石庆伟, 等. 广东地区口蹄疫病毒VP1和3A基因的序列分析[J]. 华南农业大学学报, 2018, 39(3): 13-18. DOI: 10.7671/j.issn.1001-411X.2018.03.003
    引用本文: 李琪, 谢增胜, 石庆伟, 等. 广东地区口蹄疫病毒VP1和3A基因的序列分析[J]. 华南农业大学学报, 2018, 39(3): 13-18. DOI: 10.7671/j.issn.1001-411X.2018.03.003
    shu
    LI Qi, XIE Zengsheng, SHI Qingwei, CHEN Yao, SUN Yankuo, FENG Songlin, WANG Zhi, JI Chihai, LIU Yixing, XING Xiulin, ZHANG Guihong. Sequence analysis of VP1 and 3A genes of foot-and-mouth disease virus in Guangdong area[J]. Journal of South China Agricultural University, 2018, 39(3): 13-18. DOI: 10.7671/j.issn.1001-411X.2018.03.003
    Citation: LI Qi, XIE Zengsheng, SHI Qingwei, CHEN Yao, SUN Yankuo, FENG Songlin, WANG Zhi, JI Chihai, LIU Yixing, XING Xiulin, ZHANG Guihong. Sequence analysis of VP1 and 3A genes of foot-and-mouth disease virus in Guangdong area[J]. Journal of South China Agricultural University, 2018, 39(3): 13-18. DOI: 10.7671/j.issn.1001-411X.2018.03.003
    shu

    广东地区口蹄疫病毒VP1和3A基因的序列分析

    Sequence analysis of VP1 and 3A genes of foot-and-mouth disease virus in Guangdong area

      摘要
    • 摘要:
      目的  了解广东地区口蹄疫病毒(FMDV)的遗传变异情况，为我国FMDV流行病学的研究及防控提供基础数据。
      方法  对2015—2016年间收集的广东地区猪的病料进行FMDVVP1和3A基因的扩增测序和序列分析。
      结果  11株O型FMDV均属耿马谱系毒株，7株A型FMDV均属Asia型毒株。11株O型FMDV的VP1基因与流行毒株相比，GD-MM-3株与O/BY/CHA/2010株序列相似性最高，为95.3%；GD-1株、GD-MM-1株和GD-MM-3株与O/MYA/1/98株序列相似性最高，为90.8%。7株A型FMDV的VP1基因与流行毒株相比，A/GDMM/CHA/2013株与GD-3株序列相似性最高，为99.8%；A/HuBWH/CHA/2009株与GD-3株序列相似性最高，为91.4%。11株O型FMDV的3A蛋白不存在氨基酸的缺失，7株A型FMDV的3A蛋白存在较多氨基酸的缺失。
      结论  广东部分地区FMDV基因型复杂，预防控制困难。

       

      Abstract:
      Objective  To analyze the genetic variation of foot-and-mouth disease virus (FMDV) in Guangdong area, and provide fundamental data for studying FMDV infections as well as prevention and control of foot-and-mouth disease.
      Method  We amplified and sequenced VP1 and 3A genes of FMDV from pathological samples of pigs in Guangdong area from 2015 to 2016, and analyzed the gene sequences.
      Result  Eleven strains of type O FMDV strains belonged to the social spectrum type strains. Seven type A FMDV strains belonged to the Asia type strains. Comparing the VP1 genes of the 11 type O FMDV strains with pandemic strains, GD-MM-3 and O/BY/CHA/2010 strains had the highest sequence similarity being 95.3%, while strain GD-1, GD-MM-1 and GD-MM-3 had the highest sequence similarity of 90.8% compared to strain O/MYA/1/98. Comparing the VP1 genes of 7 type A FMDV strains with pandemic strains, A/GDMM/2013 and GD-CH-3 strains had the highest sequence similarity being 99.8%, while A/HuBWH/2009 and GD-3 strains had the highest sequence similarity being 91.4%. Amino acid deletion was not found in 3A proteins of 11 type O FMDV strains. The 3A proteins of 7 type A FMDV strains had multiple amino acid deletions.
      Conclusion  The genetypes of FMDV in part area of Guangdong are complex, which leads to the difficulties of disease prevention and control.

       

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