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    徐铮, 马晓莉, 林彦星, 等. 猪圆环病毒2型重组衣壳蛋白在昆虫细胞中的表达及免疫原性分析[J]. 华南农业大学学报, 2018, 39(3): 1-5. DOI: 10.7671/j.issn.1001-411X.2018.03.001
    引用本文: 徐铮, 马晓莉, 林彦星, 等. 猪圆环病毒2型重组衣壳蛋白在昆虫细胞中的表达及免疫原性分析[J]. 华南农业大学学报, 2018, 39(3): 1-5. DOI: 10.7671/j.issn.1001-411X.2018.03.001
    shu
    XU Zheng, MA Xiaoli, LIN Yanxing, DONG Jianguo, WANG Lei, LIU Yanling, LIU Qingshen, SONG Changxu. Expression and immunogenicity of recombinant capsid protein of porcine circovirus type 2 in insect cells[J]. Journal of South China Agricultural University, 2018, 39(3): 1-5. DOI: 10.7671/j.issn.1001-411X.2018.03.001
    Citation: XU Zheng, MA Xiaoli, LIN Yanxing, DONG Jianguo, WANG Lei, LIU Yanling, LIU Qingshen, SONG Changxu. Expression and immunogenicity of recombinant capsid protein of porcine circovirus type 2 in insect cells[J]. Journal of South China Agricultural University, 2018, 39(3): 1-5. DOI: 10.7671/j.issn.1001-411X.2018.03.001
    shu

    猪圆环病毒2型重组衣壳蛋白在昆虫细胞中的表达及免疫原性分析

    Expression and immunogenicity of recombinant capsid protein of porcine circovirus type 2 in insect cells

      摘要
    • 摘要:
      目的  在昆虫细胞表达猪圆环病毒2型(PCV2)衣壳蛋白(Cap)，并对表达的重组蛋白*Cap进行免疫原性分析。
      方法  将含有PCV2-ORF2基因的重组杆状病毒质粒Bacmid-ORF2转染昆虫细胞进行蛋白表达试验，再用纯化的*Cap和质粒pcDNA3.1-ORF2分别免疫小鼠，比较二者的免疫效果。
      结果  由PCV2-ORF2基因编码的Cap结构蛋白在昆虫细胞中正确表达，相对分子质量约为32 000。*Cap能够被PCV2阳性血清所识别，具有良好的抗原性。*Cap免疫组在细胞免疫水平及体液免疫水平的免疫效果均优于pcDNA3.1-ORF2免疫组。
      结论  重组蛋白*Cap可替代全病毒，具有PCV2疫苗潜在的应用价值。研究结果为后续的PCV2病毒分子诊断、亚单位疫苗以及分子生物学等研究奠定试验基础。

       

      Abstract:
      Objective  To express the capsid protein (Cap) of porcine circovirus type 2 (PCV2) in insect cells and analyze the immunogenicity of recombinant protein.
      Method  The established recombinant bacmid containing fused ORF2 gene (Bacmid-ORF2) was transfected into insect cells for protein expression test, and then the purified *Cap and pcDNA3.1-ORF2 plasmid were immunized mice respectively to compare their immunological effects.
      Result  The Cap structural protein encoded by PCV2-ORF2gene was correctly expressed in insect cells, and the relative molecular mass was about 32 000. *Cap could be recognized by PCV2 positive serum and had good antigenicity. The immue effect of *Cap group was better than that of pcDNA3.1-ORF2 group in both cellular immunity and humoral immunity levels.
      Conclusion  The recombinant protein *Cap can replace the whole virus and may have potential application values of PCV2 vaccinum. These results lay the foundation for the study of molecular diagnosis, subunit vaccine development and molecular biology research of PCV2.

       

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