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    罗洋洋, 招丽婵, 李群辉, 等. I群禽腺病毒血清4型广东株的分离鉴定及分子特性[J]. 华南农业大学学报, 2017, 38(6): 27-33. doi: 10.7671/j.issn.1001-411X.2017.06.005
    引用本文: 罗洋洋, 招丽婵, 李群辉, 等. I群禽腺病毒血清4型广东株的分离鉴定及分子特性[J]. 华南农业大学学报, 2017, 38(6): 27-33. doi: 10.7671/j.issn.1001-411X.2017.06.005
    shu
    LUO Yangyang, ZHAO Lichan, LI Qunhui, LIN Ruiqing, WENG Yabiao. Isolation, identification and molecular characteristics of group I fowl adenovirus serotype 4 from Guangdong, China[J]. Journal of South China Agricultural University, 2017, 38(6): 27-33. DOI: 10.7671/j.issn.1001-411X.2017.06.005
    Citation: LUO Yangyang, ZHAO Lichan, LI Qunhui, LIN Ruiqing, WENG Yabiao. Isolation, identification and molecular characteristics of group I fowl adenovirus serotype 4 from Guangdong, China[J]. Journal of South China Agricultural University, 2017, 38(6): 27-33. DOI: 10.7671/j.issn.1001-411X.2017.06.005
    shu

    I群禽腺病毒血清4型广东株的分离鉴定及分子特性

    Isolation, identification and molecular characteristics of group I fowl adenovirus serotype 4 from Guangdong, China

      摘要
    • 摘要:
      目的  对广东地区疑似心包积液–肝炎综合征患病鸡进行腺病毒分离鉴定并研究其分子特征。
      方法  采集的病料样品，通过鸡胚有限稀释法经SPF鸡胚传代分离纯化病毒，进行致病性试验，并对病毒的hexon、fiber-2、ORF19和ORF29基因进行PCR扩增，分析其分子特征。
      结果  hexon基因序列分析显示此次分离的毒株为Ⅰ群禽腺病毒血清4型(命名为WM-XC株)，SPF鸡接种分离毒株后，致死率达90%，死亡鸡只出现明显的心包积液、肝炎等变化，肝脏病理切片可观察到明显的嗜酸性包涵体，显示分离毒株具有致病性。fiber-2基因序列同经典毒株ON1和KR5相应序列的相似性达96.1%和97.0%，与2015年以来国内分离的毒株序列相似性则达到100%；ORF29序列与2013年国内分离到的毒株JSJ13和JN13的相应序列相比存在33个碱基的缺失；WM-XC分离株与2015年以来国内分离毒株序列相同，但相比经典株ON1和KR-5，缺失ORF19序列。
      结论  此次从广东地区分离到的血清4型Ⅰ群腺病毒，和近年国内其他地区分离的流行毒株一样，其fiber-2、ORF19和ORF29基因序列与经典毒株差异明显。

       

      Abstract:
      Objective  To isolate and identify the pathogen causing the inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) in chicken in Guangdong Province, and study its molecular characteristics.
      Method  A virus was isolated from specific pathogen-free(SPF) chicken embryo inoculated with liver suspension from affected chickens. The virus was purified by limiting dilution method and used for pathogenicity test. Hexon, fiber-2, ORF29 and ORF19 genes were amplified by PCR, and analyzed for molecular characteristics.
      Result  Sequence analysis of hexon gene revealed the strain, named as WM-XC, belonged to group I fowl adenovirus serotype 4 (FAdV-4). This isolated strain was pathogenic based on the evidence that the mortality rate of SPF chicken reached 90% after virus inoculation, the hydropericardium-hepatitis syndrome was observed, and acidophilous inclusion body was found in the pathological section of liver. The fiber-2 gene sequence of WM-XC had 96.1% and 97.0% similarities with the corresponding sequences of ON1 and KR-5, and had 100% similarity with fiber-2 genes of fowl adenovirus isolates collected in China since 2015. There was a 33 bp deletion in ORF29 gene of WM-XC compared with JSJ13 and JN13 strains isolated in 2013 in China. Similar to isolates collected in China since 2015, WM-XC lacked ORF19 compared with classical FAdV-4 strain ON1 and KR-5.
      Conclusion  The isolated group I FAdV-4 strain and other isolates collected in China in recent years were distinct from classical FAdV-4 strains in their fiber-2, ORF19 and ORF29 gene sequences.

       

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