全二维气相色谱–飞行时间质谱分析紫藤种子提取物及其杀蚜活性研究

曹先爽; 王进; 宋丽; 姜浩; 姚曦; 汤锋; 岳永德

doi:10.7671/j.issn.1001-411X.2017.05.012

全二维气相色谱–飞行时间质谱分析紫藤种子提取物及其杀蚜活性研究

GC×GC-TOF/MS analysis of the seed extract of Wisteria sinensis and the insecticidal activity

摘要

摘要:
目的通过对紫藤Wisteria sinensis种子提取物的化学成分分析及杀蚜活性测定和抗氧化活性评价，为紫藤种子资源在植物源农药领域的利用提供基础。

方法以紫藤种子提取物为材料，采用全二维气相色谱–飞行时间质谱(GC×GC-TOF/MS)技术进行成分分析，以微量点滴法开展对棉蚜Aphis gossypii的触杀毒力测定，用2,2–二苯基–1–苦基肼(DPPH)自由基清除法进行抗氧化活性评价。

结果从紫藤种子石油醚提取物中检测到61种化合物，主要包括酯类、烷烃类、芳香烃类等化合物，其中相对含量大于9%的化合物有：对二甲苯(14.33%)、十一烷(11.89%)、1,4–二乙基苯(11.02%)和癸烷(9.54%)。紫藤种子提取物对棉蚜的致死中质量浓度(LC₅₀)为193.22 mg·L^–1(处理后24 h)。同时，紫藤种子提取物具有抗氧化活性，对DPPH的抑制中质量浓度(IC₅₀)为4.15 g·L^–1。

结论紫藤种子提取物具有较强的杀蚜活性，有望作为植物源杀蚜剂用于棉蚜的防治。

Abstract:
Objective To analyze the chemical composition, insecticidal and antioxidant activities of the seed extract of Wisteria sinensis, and provide a basis for developing botanical pesticide from W. sinensis germplasm resources.

Method The seed extract of W. sinensis was analyzed by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF/MS). The toxicity against Aphis gossypii was determined by topical application bioassay. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method.

Result A total of 61 components were identified from the seed extract of W. sinensis, mainly including esters, alkane, arene, etc. The constituents with higher than 9% relative content were p-xylene (14.33%), undecane (11.89%), 1,4-diethyl-benzene (11.02%) and decane (9.54%). The seed extract of W. sinensis showed insecticidal activity against A. gossypii with the LC₅₀ value of 193.22 mg·L^–1 after 24 h treatment, and also showed antioxidant activity with the IC₅₀ value of 4.15 g·L^–1 against DPPH.

Conclusion The seed extract of W. sinensis has strong insecticidal activity, and has the potential to be developed into a natural insecticide for controlling A. gossypii.

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