腹腔注射亚精胺对小鼠卵巢组织多胺含量及代谢相关基因表达的影响

陈咨余; 姜冬梅; 康波; 管成; 龙诗韵; 易治鑫; 徐麒麟

doi:10.7671/j.issn.1001-411X.2017.04.009

腹腔注射亚精胺对小鼠卵巢组织多胺含量及代谢相关基因表达的影响

Effects of intraperitoneal spermidine injection on polyamine content and metabolism-related gene expression in mouse ovary

摘要

摘要:
目的研究腹腔注射亚精胺对鼠卵巢组织多胺代谢的影响。
方法给鼠腹腔注射不同剂量亚精胺0(对照组、0.05、0.10和0.15 mg·g^-1，应用实时荧光定量PCR检测多胺代谢关键基因表达量, 应用高效液相色谱检测鼠卵巢组织中多胺含量。
结果注射0.15 mg·g^-1亚精胺时，卵巢组织ODC、OAZ1、SPMS、SSAT、PAOX和SMOX基因表达量显著高于其他3组；注射亚精胺组鼠卵巢组织SPDS表达量均显著低于对照组；注射0.10 mg·g^-1亚精胺组鼠卵巢组织中腐胺和亚精胺含量均显著高于对照组，而注射亚精胺对卵巢中精胺含量无显著影响。
结论外源性亚精胺的腹腔注射可导致鼠卵巢组织中多胺含量以及多胺代谢相关基因表达量发生变化，且具有剂量依赖性，提示外源性亚精胺可通过介导卵巢组织的多胺代谢来参与调控卵巢功能。

Abstract:
Objective To observe the effects of intraperitoneal injection of spermidine on polyamine metabolism in mouse ovary.
Method Mice were injected with different doses of spermidine0(control), 0.05, 0.10 and 0.15 mg·g^-1. Real-time fluorescent quantitative PCR was used to determine the ovarian expression levels of key genes in polyamine metabolism. High performance liquid chromatography (HPLC) was used to detect the polyamine levels in mouse ovarian tissues.
Result After administration of 0.15 mg·g^-1 spermidine, the expression of ODC, OAZ1, SPMS, SSAT, PAOX and SMOX genes in ovarian tissues were significantly higher compared to other three groups. SPDS gene expressions of all three spermidine administration groups were significantly lower compared to control. After administration of 0.10 mg·g^-1 spermidine, the putrescine and spermidine contents in mouse ovary were significantly higher compared to control, while spermidine administration had no significant effect on spermine content in ovary.
Conclusion Exogenous spermidine injected intraperitoneally can change polyamine content and polyamine metabolism-related gene expression in mouse ovary. Such effects are dose-dependent. Exogenous spermidine can potentially participate in the regulation of ovarian function through adjusting polyamine metabolism.

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