印楝素干粉的制备及生物活性测定

李一鸣; 陈孝甜; 王勇庆; 程东美; 张志祥

doi:10.7671/j.issn.1001-411X.2017.04.008

印楝素干粉的制备及生物活性测定

Preparation and bioactivity determination of azadirachtin-rich dry powder

摘要

摘要:
目的测定印楝素干粉及印楝素A、印楝素B的生物活性，建立印楝素干粉中印楝素含量的检测方法。
方法采用乙酸乙酯浸提法制备印楝素干粉，HPLC法检测印楝素含量，叶碟法测定生物活性。
结果处理24 h后，印楝素干粉对斜纹夜蛾Spodoptera litura 2龄幼虫和大菜粉蝶Pieris brassicae 3龄幼虫的AFC₅₀值分别为0.28和70.71 μg·mL^-1，拒食活性明显高于印楝素A和印楝素B。
结论该干粉制备方法不仅简单有效，且生物活性良好，具有推广应用价值。

Abstract:
Objective To study the biological activity of azadirachtin-rich dry powder, azadirachtin A and azadirachtin B, and establish a method for determinating azadirachtin content of dry powder.
Method Azadirachtin-rich dry powder was prepared by ethyl acetate extraction method, the azadirachtin content of dry powder was determined by HPLC and the biological activity was measured by leaf disc test.
Result AFC₅₀ of zadirachtin-rich dry powder was 0.28 μg·mL^-1 against 2nd instar larvae of Spodoptera litura and 70.71 μg·mL^-1 against the 3rd instar larvae of Pieris brassicae 24 h after treatment. The antifeedant activity of azadirachtin-rich dry powder was clearly higher than that of azadirachtin A and azadirachtin B.
Conclusion This preparation method of the dry powder is simple, effective, and has good biological activity. It is recommended for popularization and application.

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