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    燕颍, 顾怀宇, 徐汉虹, 张志祥. 印楝素对果蝇厌恶性味觉记忆的诱导及多巴胺能神经元的影响[J]. 华南农业大学学报, 2017, 38(4): 12-18. DOI: 10.7671/j.issn.1001-411X.2017.04.002
    引用本文: 燕颍, 顾怀宇, 徐汉虹, 张志祥. 印楝素对果蝇厌恶性味觉记忆的诱导及多巴胺能神经元的影响[J]. 华南农业大学学报, 2017, 38(4): 12-18. DOI: 10.7671/j.issn.1001-411X.2017.04.002
    shu
    YAN Ying, GU Huaiyu, XU Hanhong, ZHANG Zhixiang. Induction of aversive taste memory by azadirachtin and its effects on dopaminergic neurons of Drosophila[J]. Journal of South China Agricultural University, 2017, 38(4): 12-18. DOI: 10.7671/j.issn.1001-411X.2017.04.002
    Citation: YAN Ying, GU Huaiyu, XU Hanhong, ZHANG Zhixiang. Induction of aversive taste memory by azadirachtin and its effects on dopaminergic neurons of Drosophila[J]. Journal of South China Agricultural University, 2017, 38(4): 12-18. DOI: 10.7671/j.issn.1001-411X.2017.04.002
    shu

    印楝素对果蝇厌恶性味觉记忆的诱导及多巴胺能神经元的影响

    Induction of aversive taste memory by azadirachtin and its effects on dopaminergic neurons of Drosophila

      摘要
    • 摘要:
      目的 明确印楝素是否能诱导果蝇产生厌恶性味觉记忆, 并探讨多巴胺信号在这种记忆形成中的调控作用。
      方法 利用印楝素诱导果蝇产生短期厌恶性味觉记忆，并通过昆虫口器伸展反应测试诱导结果；采用压力注射给药方式及果蝇全脑膜片钳记录，研究印楝素对果蝇脑内不同亚群多巴胺能神经元兴奋性及受体电流的影响。
      结果 印楝素A及印楝素干粉均能显著抑制果蝇口器伸展的概率，口器伸展反应(PER)分别为60.34%和17.24%，(P < 0.007)，并且干粉的效果更加明显；印楝素对不同亚群的多巴胺能神经元的兴奋性有不同的作用，PPL1、PAM和PPM2亚群兴奋性呈现增加趋势，其中PPL1亚群兴奋性改变最为显著；印楝素对多巴胺D1受体具有激动效应，这种激动效应可被D1受体特异性拮抗剂抑制。
      结论 印楝素可以诱导果蝇产生厌恶性味觉记忆，这种记忆受果蝇脑内多巴胺能信号的调控。

       

      Abstract:
      Objective  To investigate the inductive effects of azadirachtin on taste memory in Drosophila melanogaster (fruit fly) and the regulation of dopamine signaling in such memory formation.
      Method Aversive taste memory induced by azadirachtin was tested by proboscis extension reflex (PER). Effects of azadirachtin on membrane excitability and receptor currents of dopaminergic neurons in D. melanogaster brain were studied using pressure injection combined with whole-cell patch clamp recording.
      Result Both azadirachtin A and the azadirachtin dry powder could significantly inhibit the probability of PER, which were 60.34% and 17.24% respectively(P < 0.007). The effect of dry powder was more obvious. Different clusters of dopaminergic neurons responded variously to azadirachtin. PPL1, PAM and PPM2 subgroups showed increasing trends in excitability, in which the changes of PPL1 cluster neurons were most pronounced. Azadirachtin showed agonistic effect on D1 receptor, and such effect could be inhibited by D1 receptor specific antagonists.
      Conclusion Azadirachtin can induce aversive taste memory in Drosophila, and such memory is regulated by dopaminergic signals in the brain.

       

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