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    林玮, 周玮, 周鹏, 周祥斌, 吴林瑛, 陈晓阳. 基于SRAP标记的任豆遗传多样性分析[J]. 华南农业大学学报, 2017, 38(1): 82-89. DOI: 10.7671/j.issn.1001-411X.2017.01.014
    引用本文: 林玮, 周玮, 周鹏, 周祥斌, 吴林瑛, 陈晓阳. 基于SRAP标记的任豆遗传多样性分析[J]. 华南农业大学学报, 2017, 38(1): 82-89. DOI: 10.7671/j.issn.1001-411X.2017.01.014
    shu
    LIN Wei, ZHOU Wei, ZHOU Peng, ZHOU Xiangbin, WU Linying, CHEN Xiaoyang. Genetic diversity of Zenia insignis based on SRAP markers[J]. Journal of South China Agricultural University, 2017, 38(1): 82-89. DOI: 10.7671/j.issn.1001-411X.2017.01.014
    Citation: LIN Wei, ZHOU Wei, ZHOU Peng, ZHOU Xiangbin, WU Linying, CHEN Xiaoyang. Genetic diversity of Zenia insignis based on SRAP markers[J]. Journal of South China Agricultural University, 2017, 38(1): 82-89. DOI: 10.7671/j.issn.1001-411X.2017.01.014
    shu

    基于SRAP标记的任豆遗传多样性分析

    Genetic diversity of Zenia insignis based on SRAP markers

      摘要
    • 摘要:
      目的 研究任豆Zenia insignis种群遗传多样性，为有效保护任豆种质资源并进行遗传改良提供理论基础。
      方法 在建立任豆SRAP-PCR反应体系的基础上，对17个任豆种源进行遗传多样性分析，并利用UPGMA聚类分析，对任豆种源进行类群划分。
      结果 12对引物组合共扩增出151条带，平均每对引物获得12.58条。其中，多态性条带106条，平均每对引物8.83条，平均多态率为70.39%。种源间多态位点比率为38.96%~72.73%，平均为59.66%；基因多样性指数为0.175 5~0.313 3，平均为0.256 8；Shannon信息指数为0.249 4~0.450 2，平均为0.369 1；观测等位基因数(na)为1.519 5~1.727 3，平均达1.600 0，种源水平的na为1.724 9；有效等位基因数(ne)为1.330 5~1.577 3，平均达1.471 3，种源水平的ne为1.502 6；种源间的遗传一致度为0.703 1~0.886 5；遗传距离为0.120 5~0.352 3。根据聚类结果，将任豆17个种源分为3个地理类群：第1类为广西和贵州种源，第2类为广东、湖南和广西种源，第3类为云南种源，地理距离相近的种源基本上聚在同一类。
      结论 任豆种源间和种源个体间均存在较丰富的遗传多样性，且种源内更丰富，遗传改良时应更注重种源内个体的选择。任豆种源间基因流不高，且根据聚类结果划分的3个类群的地理格局明显，应是由任豆特定的生活环境造成的隔离所致。

       

      Abstract:
      Objective To study population genetic diversity of Zenia insignis, and to provide a basis for Z. insignis germplasm protect and promote genetic improvement.
      Method Base on establishing of SRAP-PCR system in Z. insignis, the genetic diversity among 17 provenances was analyzed. UPGMA clustering analysis was used to divide Z. insignis provenances into different groups.
      Result A total of 151 bands were amplified from 12 primer pairs, and in average 12.58 bands were amplified from each primer pair. There were 106 polymorphic bands, in average 8.83 bands per primer sets, and the average percentage of polymorphic bands was 70.39%. The ratios of polymorphic loci among provenances were 38.96%-72.73%, and 59.66% in average. The genetic diversity indices were 0.175 5-0.313 3, and 0.256 8 in average. The Shannon information indices were 0.249 4-0.450 2, and 0.369 1 in average. The numbers of alleles (na) observed were 1.519 5-1.727 3, and 1.600 0 in average. The number of alleles (na) at the provenance level was 1.724 9. The numbers of effective alleles (ne) were 1.330 5-1.577 3, and 1.4713 in average. The number of effective alleles (ne) at the provenance level was 1.502 6. The genetic identity degrees among provenances were 0.703 1-0.886 5.The genetic distances were 0.120 5-0.352 3. According to cluster analysis, 17 provenances were divided into three groups. The first group included Guangxi and Guizhou provenances. The second group included Guangdong, Hunan and Guangxi provenances. The third group only included Yunnan provenance. The provenances with geographic proximity were generally clustered into the same group.
      Conclusion The genetic diversity is abundant among Z. insignis provenances and among individuals within provenance, but is mainly from individuals within provenance. Therefore more attention should be paid to individuals in genetic improvement of Z. insignis. Both the low level of gene flow among provenances and three clear geographic clustering should be caused by the geographic isolation due to the specific living environment of Z.insignis.

       

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