高级检索
    李福霞, 胡维娜, 胡琼波. 球孢白僵菌对亚洲玉米螟血细胞毒力和形态的影响[J]. 华南农业大学学报, 2016, 37(4): 32-37. DOI: 10.7671/j.issn.1001-411X.2016.04.006
    引用本文: 李福霞, 胡维娜, 胡琼波. 球孢白僵菌对亚洲玉米螟血细胞毒力和形态的影响[J]. 华南农业大学学报, 2016, 37(4): 32-37. DOI: 10.7671/j.issn.1001-411X.2016.04.006
    shu
    LI Fuxia, HU Weina, HU Qiongbo. Effects of Beauveria bassiana on virulence and cellular morphology of hemocytes of Ostrinia furnacalis[J]. Journal of South China Agricultural University, 2016, 37(4): 32-37. DOI: 10.7671/j.issn.1001-411X.2016.04.006
    Citation: LI Fuxia, HU Weina, HU Qiongbo. Effects of Beauveria bassiana on virulence and cellular morphology of hemocytes of Ostrinia furnacalis[J]. Journal of South China Agricultural University, 2016, 37(4): 32-37. DOI: 10.7671/j.issn.1001-411X.2016.04.006
    shu

    球孢白僵菌对亚洲玉米螟血细胞毒力和形态的影响

    Effects of Beauveria bassiana on virulence and cellular morphology of hemocytes of Ostrinia furnacalis

      摘要
    • 摘要:
      目的 测定球孢白僵菌 Beauveria bassiana 对亚洲玉米螟 Ostrinia furnacalis 血细胞系SYSU-OfHe-C的影响, 为促进球孢白僵菌在防治亚洲玉米螟中的应用提供理论依据。
      方法 采用MTT和CCK-8法测定球孢白僵菌对亚洲玉米螟血细胞系SYSU-OfHe-C的毒力, 通过光学显微镜与扫描电镜观察球孢白僵菌生长及其对SYSU-OfHe-C细胞的影响。
      结果 球孢白僵菌分生孢子处理SYSU-OfHe-C细胞24 h后, MTT法的IC50值为2.8×105 mL-1, CCK-8法的IC50值为1.4×105 mL-1。光学显微镜实时监测发现, 在SYSU-OfHe-C细胞对数期接种球孢白僵菌浓度为3.5×104 mL-1时, 球孢白僵菌具有明显竞争力, 未发现囊胞化与吞噬等细胞免疫现象; 处理10 h后, 球孢白僵菌分生孢子开始萌发长出菌丝, 而SYSU-OfHe-C细胞减少且开始变形; 处理24 h, 球孢白僵菌几乎长满整个视野; SYSU-OfHe-C细胞从近似圆形变长或者变为不规则形状, 出现聚集、破碎、胞内空泡、瘤状突起, 以及被白僵菌菌丝穿透等现象。
      结论 球孢白僵菌可改变亚洲玉米螟血细胞系SYSU-OfHe-C的细胞形态, 但不能使其发生细胞免疫现象。

       

      Abstract:
      Objective To test the effect of Beauveria bassiana on SYSU-OfHe-C, a cell line from hemocytes of Ostrinia furnacalis. To provide a theory basis for application of B.bassiana in the prevention and treatment of O.furnacalis.
      Method MTT and CCK-8 were employed to test the virulence of B.bassiana on SYSU-OfHe-C.Optical microscope and scanning electron microscope were used to observe the growth of B.bassiana and its influence on the morphology of SYSU-OfHe-C cells.
      Result The IC50 values of B.bassiana against SYSU-OfHe-C cells at 24 hours after treatment were recorded as 2.8×105 and 1.4×105 mL-1 respectively by MTT and CCK-8.Furthermore, under real-time monitoring with optical microscope, encapsulization and phagocytosis were not found and B.bassiana was highly competitive when the conidia of B.bassiana were inoculated in the culture of SYSU-OfHe-C cells at logarithmic stage reaching a final concentration of 3.5×104 mL-1 for B.bassiana.B.bassiana started to germinate at 10 hours after treatment, and meanwhile, SYSU-OfHe-C cells started to reduce in number and change in morphology.At 24 hours after treatment, B.bassiana grew the entire visual field.The nearly round SYSU-OfHe-C cells had elongated or turned into irregular shape.Aggregation, fragmentation, cavitation, protuberance and penetration of SYSU-OfHe-C cells caused by B.bassiana mycelia were observed.
      Conclusion B.bassiana can change cellular morphology of hemocytes of O.furnacalis, but cannot induce its immune responses.

       

      • HTML全文
      • 参考文献(16)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回