1株解淀粉芽孢杆菌HN011抑菌次级代谢产物的分析

张龙来; 康向辉; 魏孝义; 徐汉虹

doi:10.7671/j.issn.1001-411X.2016.01.011

1株解淀粉芽孢杆菌HN011抑菌次级代谢产物的分析

Research on secondary metabolites from Bacillus amyloliquefaciens strain HN011

摘要

摘要:
目的分离和鉴定生防菌HN011在YPD条件下发酵的次级代谢产物。
方法 HN011少量(1 L)发酵，分别用石油醚、氯仿、乙酸乙酯、正丁醇进行液-液萃取，通过金黄色葡萄球菌Staphylococcus aureus的活性试验跟踪确定活性部位; 通过放大发酵，对活性部位进行分离得到单体化合物。利用核磁共振和质谱等手段，鉴定单体化合物。
结果生防菌HN011少量发酵后，发酵液经不同极性的有机溶剂液-液萃取，萃取物的活性试验表明，乙酸乙酯部位活性最强，其次氯仿部位，正丁醇部位活性较弱，石油醚部位、水部位活性不明显。通过放大发酵，在活性部位分离出15个单体，结构鉴定表明，主要为小分子的环二肽。
结论生防菌HN011在YPD的发酵条件下，次级代谢产物主要为一些小分子的环二肽。

Abstract:
Objective To separate and identify secondary metabolites of bio-control bacterium strain HN011 in YPD zymotic fluid.
Method By a small amount of fermentation (1 L), the zymotic fluid was extracted by using liquid-liquid extraction of petroleum ether, chloroform, ethyl acetate and n-butanol. The active portions were determined by Staphylococcus aureus bioassay. The pure compounds were isolated from the active portions by amplifying fermentation. The pure compounds were identified by NMR and mass spectrometry.
Result The bioassay results showed that the activities of different solvent extracts from the zymotic fluid were different. The most obvious activity was ethyl acetate portion, followed by chloroform. The activity of n-butanol portion was weak, and petroleum ether and water portions were not obvious. By amplifying fermentation, fifteen pure compounds were isolated from the bioassay portions, and identified as mainly small molecule cyclic dipeptides based on their structures.
Conclusion The secondary metabolites, which are produced by bio-control bacterium strain HN011 in YPD zymotic fluid, are mainly small molecule cyclic dipeptides.

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