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    陈炎, 赵俊龙, 毛根林, 汪聪颖, 林菲, 徐汉虹, 朱小源. 三环唑诱导水稻抗性相关基因的表达分析[J]. 华南农业大学学报, 2016, 37(1): 35-40. DOI: 10.7671/j.issn.1001-411X.2016.01.006
    引用本文: 陈炎, 赵俊龙, 毛根林, 汪聪颖, 林菲, 徐汉虹, 朱小源. 三环唑诱导水稻抗性相关基因的表达分析[J]. 华南农业大学学报, 2016, 37(1): 35-40. DOI: 10.7671/j.issn.1001-411X.2016.01.006
    shu
    CHEN Yan, ZHAO Junlong, MAO Genlin, WANG Congying, LIN Fei, XU Hanhong, ZHU Xiaoyuan. Tricyclazole induced expression of genes associated with rice resistance[J]. Journal of South China Agricultural University, 2016, 37(1): 35-40. DOI: 10.7671/j.issn.1001-411X.2016.01.006
    Citation: CHEN Yan, ZHAO Junlong, MAO Genlin, WANG Congying, LIN Fei, XU Hanhong, ZHU Xiaoyuan. Tricyclazole induced expression of genes associated with rice resistance[J]. Journal of South China Agricultural University, 2016, 37(1): 35-40. DOI: 10.7671/j.issn.1001-411X.2016.01.006
    shu

    三环唑诱导水稻抗性相关基因的表达分析

    Tricyclazole induced expression of genes associated with rice resistance

      摘要
    • 摘要:
      目的 探讨三环唑处理下水稻抗性相关基因表达的动态变化及与抗瘟性的相关性。
      方法 室内抑菌活性测定三环唑对稻瘟病菌丝生长和孢子萌发的抑制作用；温室不同时间点接种稻瘟病菌后施用三环唑，测定其防效；利用实时荧光定量PCR对水稻受三环唑处理后不同时间点诱导抗性相关基因进行表达分析。
      结果 三环唑对水稻稻瘟菌菌丝生长和孢子萌发无显著抑制作用；接种稻瘟病菌24 h后施用三环唑，仍然能够获得87.2%的防治效果。RT-qPCR结果表明，三环唑能够诱导水稻防卫反应基因 OsNH1-1 、OsPR1a和OsPR10的表达，进一步选取水杨酸和茉莉酸途径的关键基因进行检测，发现茉莉酸途径的关键基因OsLOX和OsAOS2的表达受到三环唑显著诱导。
      结论 三环唑防治稻瘟病并非仅仅抑制了黑色素的合成，三环唑对水稻具有诱导抗性作用，三环唑主要通过茉莉酸途径起诱导抗性作用。

       

      Abstract:
      Objective This study aims at analyzing both the dynamics and the correlations with rice blast resistance of rice defense related gene expression under tricyclazole treatment.
      Method Antifungal activity of tricyclazole was determined indoor under greenhouse conditions. Real-time PCR analysis was performed to monitor the expression levels of rice defense related genes after tricyclazole treatment.
      Result Rice blast fungus mycelium growth and spore germination were not inhibited by tricyclazole. However, tricyclazole treatment 24 h after rice blast fungus inoculation could still help 87.2% of the rice strains to successfully defense the fungi. RT-qPCR results showed that rice defense related genes OsNH1-1, OsPR1a and OsPR10 were induced by tricyclazole. Moreover, by analyzing the expression of key genes in the salicylic acid and jasmonic acid pathways, the key genes of OsLOX and OsAOS2 from the latter one were significantly induced by tricyclazole treatment.
      Conclusion The prevention of rice blast by tricyclazole is not only by inhibiting the synthesis of melanin. RT-qPCR results show that tricyclazole can trigger rice resistance by inducing expression of key genes in the jasmonic acid pathway.

       

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