基于主成分分析和聚类分析的红火蚁毒腺生物碱的比较研究

张文倩; 胡林; 陈立; 曾任森

doi:10.7671/j.issn.1001-411X.2015.06.012

基于主成分分析和聚类分析的红火蚁毒腺生物碱的比较研究

张文倩^{1, 2,},
胡林²,
陈立²,
曾任森^1, ,

1.
华南农业大学农学院, 广东广州 510642
2.
中国科学院动物研究所/农业虫害鼠害综合治理研究国家重点实验室, 北京 100101

基金项目:

国家自然科学基金 30970402

详细信息

作者简介:
张文倩(1990-), 女, 硕士研究生, E-mail:woshizwq@126.com

通讯作者:
曾任森(1965-), 男, 教授, 博士, E-mail:rszeng@scau.edu.cn

中图分类号: S481
计量
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出版历程
- 收稿日期: 2014-12-22
- 网络出版日期: 2023-05-17
- 刊出日期: 2015-11-09

Comparative studies on alkaloids secreted by vemon gland of Solenopsis invicta based on principal component analysis and cluster analysis

ZHANG Wenqian^{1, 2,},
HU Lin²,
CHEN Li²,
ZENG Rensen^1, ,

1.
College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2.
State Key Laboratory of Integrated Management of Pest Insects and Rodents/Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

摘要

摘要:
目的
分析中国和美国2个入侵地红火蚁Solenopsis invicta毒腺生物碱的主要化学成分及其聚类特征.
方法
以中国广州、惠州、深圳、珠海和东莞以及美国阿拉巴马州采样地共24巢红火蚁为样本, 提取各个蚁巢毒腺的生物碱流分, 分别以红火蚁顺式生物碱流分中的15种生物碱和反式生物碱流分中的7种生物碱相对含量作为变量, 建立2个数据矩阵, 进行主成分分析和聚类分析.
结果和结论
主成分分析结果表明, 将红火蚁顺式生物碱流分中的15种生物碱综合成为2个主成分因子, 主要是顺式-2-甲基-6-(4′-十三烯)哌啶、2-甲基-6-(6′-十五烯)-Δ^{1, 6}-哌啶、2-甲基-6-十五烷基-Δ^{1, 6}-哌啶和顺式-2-甲基-6-十一烷基哌啶; 反式生物碱流分中的7种生物碱综合成1个主成分因子, 主要是反式-2-甲基-6-(4′-十三烯)哌啶和反式-2-甲基-6-(6′-十五烯)哌啶.顺式生物碱流分的聚类分析可以将美国种群与中国种群区分开来, 而反式生物碱流分不能.
- 红火蚁 /
- 毒腺 /
- 生物碱 /
- 主成分分析 /
- 聚类分析
Abstract:
Objective
To analyze the major components and cluster features of alkaloids, which are secreted by vemon gland of red imported fire ants, Solenopsis invicta, from China and USA.
Method
Twenty four colonies of S.invicta were collected from Guangzhou, Huizhou, Shenzhen, Zhuhai, Dongguan of China and Alabama of USA.Alkaloids of vemon gland were extracted.Two data matrix were established with variable of relative contents of 15 kinds of cis-alkaloids and 7 kinds of trans-alkaloid respectively.The alkaloid fraction was analyzed by principal component analysis and cluster analysis.
Result and conclusion
The results showed that the 15 types of alkaloids from the cis-alkaloids fraction were consolidated into two principal components, including cis-2-methyl-6-(4′-tridecenyl) piperidine, 2-methyl-6-(6′-pentadecenyl) -Δ^{1, 6}-piperidine, 2-methyl-6-pentadecyl-Δ^{1, 6}-piperidine and cis-2-methyl-6-undecylpiperidine seven types of alkaloids from the trans-alkaloid fraction were consolidated into one principal component, including trans-2-methyl-6-(4′-tridecenyl) piperidine and trans-2-methyl-6-(6′-pentadecenyl) piperidine.The results of cluster analyses demonstrate that alkaloids from the cis-alkaloids fraction can distinguish the USA populations from the Chinese populations, but alkaloids from the trans-alkaloid fraction can not.
- Solenopsis invicta /
- vemon gland /
- alkaloid /
- principal component analysis /
- cluster analysis

HTML全文

图 1 红火蚁工蚁顺式和反式生物碱流分总离子色谱图

A:1 ~ 15分别对应顺式-2-甲基-6-十一烷基哌啶、顺式-2-甲基-6-(4′-十三烯)哌啶、2-甲基-6-(4′-十三烯)-Δ^{1, 6}-哌啶、顺式-2-甲基-6-十三烷基哌啶、2-甲基-6-十三烷基-Δ^{1, 6}-哌啶、2-甲基-6-(4′-十三烯)-Δ^{1, 2}-哌啶、2-甲基-6-十三烷基Δ^{1, 2}-哌啶、顺式-2-甲基-6-(6′-十五烯)哌啶、2-甲基-6-(6′-十四烯)-Δ^{1, 6}-哌啶、顺式-2-甲基-6-十五烷基哌啶、2-甲基-6十五烷基-Δ^{1, 6}-哌啶、顺式-2-甲基-6-(8′-十七烯)哌啶、2-甲基-6-(8′-十七烯)-Δ^{1, 6}-哌啶、顺式-2-甲基-6-十七烷基哌啶、2-甲基-6-十七烷基-Δ^{1, 6}-哌啶; B:1 ~ 7分别对应反式-2-甲基-6-十一烷基哌啶、反式-2-甲基-6-(4′-十三烯)哌啶、反式-2-甲基-6-十三烷基哌啶、反式-2-甲基-6-(6′-十五烯)哌啶、反式-2-甲基-6-十五烷基哌啶、反式-2-甲基-6-(8′-十七烯)哌啶、反式-2-甲基-6-十七烷基哌啶.

Figure 1. Total ion chromatograms of the cis- and trans- alkaloid fractions extracted from red imported fire ant workers from different areas

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图 2 红火蚁顺式和反式生物碱流分的聚类分析树状图

DG表示东莞, ZH表示珠海, SZ表示深圳, GZ表示广州, HZ表示惠州, US表示美国; 1、2、3、4分别表示在采样地采集的不同蚂蚁巢穴.

Figure 2. Dendrogram generated from clustering analysis of 15 compounds from the cis-alkaloid fraction and 7 compounds from the trans-alkaloid fraction of red imported fire ants

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表 1 顺式生物碱流分的主成分分析

Table 1 Principal component analysis of the cis-alkaloid fraction

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表 2 顺式生物碱流分各化合物的载荷矩

Table 2 Component matrix of 15 compounds from the cis-alkaloid fraction

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表 3 反式生物碱流分的主成分分析

Table 3 Principal component analysis of the trans-alkaloid fraction

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表 4 反式生物碱流分各化合物的载荷矩

Table 4 Component matrix of 7 compounds from the trans-alkaloid fraction

下载: 导出CSV

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